Nuclear Extraction Protocol
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实验试剂
Cell Extraction Buffer (Cat. no. FNN0011)
Protease Inhibitor Cocktail, Sigma (Cat. no. P-2714)
Quant-iT™ Protein Quantitation Kit (Cat. no. Q33210)
Cell Extraction Buffer Formulation
- 100 mM Tris, pH 7.4
- 2 mM Na3 VO4
- 100 mM NaCl
- 1% Triton X-100
- 1 mM EDTA
- 10% glycerol
- 1 mM EGTA
- 0.1% SDS
- 1 mM NaF
- 0.5% deoxycholate
- 20 mM Na4 P2 O7
实验设备
实验材料
实验步骤