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3. Lyse cells on ice for 10 minutes with gentle stirring.
5. Wash the nuclei in half the volume of TEB and centrifuge as before.
6. Re-suspend the pellet in 0.2 N HCl at a density of 4x107 nuclei per ml.
7. Acid extract the histones over night at 4°C.
8. Centrifuge samples at 6,500 x g for 10 minutes at 4°C to pellet debris.
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