Nuclear fractionation protocol
互联网
2253
实验试剂
2. Buffer B
5 mM HEPES, 1.5 mM MgCl2 , 0.2 mM EDTA, 0.5 mM DTT,
26% glycerol (v/v), pH 7.9
To prepare 250 ml stock of buffer B:
3. 4.6 M NaCl – 87.66 g/326 ml
实验步骤
3. Centrifuge at 4°C at 3000 rpm for 10 min.
6. Homogenize with 20 full strokes in Dounce or glass homogenizer on ice.
8. Centrifuge at 24,000 g for 20 min at 4°C.
9. Aliquot supernatant, remove 10 μl for Protein Quantification (ab102536) and store at -70°C.
For your convenience, please consider some of our cell fractionation kits such as ab109718.
