Affinity Measurement Using Surface Plasmon Resonance
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The change in free energy for a reaction, ΔG, can be determined from the dissociation affinity constant, K D , since ΔG is equal to RTln K D . The affinity constant provides no information on the rate of the reaction or the energy required to reach the transition state of the reaction (Fig. 1 ).
Fig. 1. Energy vs reaction coordinates. A=analyte, L=ligand.
