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The Construction of Bacterial Artificial Chromosome (BAC) Libraries (complete manuscript) (Clemson University Genomics Institute)
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Construction of BAC Library (Caltech)
Guideline for BAC library preparation, the followings are described in great detail- Preparation of BAC vector DNA
- Preparation of source DNA
- Ligation and Electroporation
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Preparation of BAC DNA
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BAC Manual (Texas A&M BAC Center)
Provide details in the theory and practice for the construction, characterization, and screening of BAC libraries.- Introduction
- MegaBase DNA Preparation
- BAC Vector Preparation
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Solutions
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Hybridization of High Density Arrayed BAC Nylon Filter Blots (Caltech)
Procedures for isolation and purification of cDNA inserts using polymerase chain reaction, probe labeling, probe hybridization and more...
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BAC/PAC/Fosmid Prep (WUGSC)
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BAC Sequencing with ET primers (WUGSC)
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BAC End-Sequencing (Caltech)
Prepare BAC DNA for sequencing
- Large Scale Plasmid, Cosmid, BAC, PAC, and Fosmid DNA Isolation by a Cleared Lysate Method Followed by Double Acetate Precipitation (Roe Lab)
