帮我设计一下引物探针好吗?
丁香园论坛
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我准备用Tagman探针做real time PCR,帮我设计一下好吗?我要设计三组,检测的是两个基因, 内参用P-actin,或GAPDH, 最好能参照你用过的内参引物探针.谢谢
各位大侠快帮忙啊!急
1)
RNA (U50930.1:645..772, U50931.1604..1837)
CDS U50930.1:712..772, U50931.1604..1749)
agctcag cctccaaagg
661 agccagcctc tccccagttc ctgaaatcct gagtgttgcc tgccagtcgc catgagaact
721 tcctaccttc tgctgtttac tctctgctta cttttgtctg agatggcctc ag
gtggtaa ctttctcaca
1621 ggccttggcc acagatctga tcattacaat tgcgtcagca gtggagggca atgtctctat
1681 tctgcctgcc cgatctttac caaaattcaa ggcacctgtt acagagggaa ggccaagtgc
1741 tgcaagtgag ctgagagtga ccagaagaaa tgacgcagaa gtgaaatgaa ctttttataa
1801 gcattctttt aataaaggaa aattgctttt gaagtat
编码蛋白MRTSYLLLFTLCLLLSEMASGGNFLTGLGHRSDHYNCVSSGGQCLYSACPIFTKIQGTCYRGKAKCCK
全长363bp 内含子:位于129-130bp间,全长6963bp
在基因组中的序列(70698-70821 63502-63735)(互补链上)
2)
RNA2258..2338,4020..4257)
CDS2281..2338,4020..4156)
ggt gaagctccca gccatcagcc
2281 atgagggtct tgtatctcct cttctcgttc ctcttcatat tcctgatgcc tcttccag
g
4021 tgtttttggt ggtataggcg atcctgttac ctgccttaag agtggagcca tatgtcatcc
4081 agtcttttgc cctagaaggt ataaacaaat tggcacctgt ggtctccctg gaacaaaatg
4141 ctgcaaaaag ccatgaggag gccaagaagc tgctgtggct gatgcggatt cagaaagggc
4201 tccctcatca gagacgtgcg acatgtaaac caaattaaac tatggtgtcc aaagata
编码蛋白:MRVLYLLFSFLFIFLMPLPGVFGGIGDPVTCLKSGAICHPVFCPRRYKQIGTCGLPGTKCCKKP
全长:319bp 内含子:位于81bp-82bp间 ,长1682bp
各位大侠快帮忙啊!急
1)
RNA (U50930.1:645..772, U50931.1604..1837)
CDS U50930.1:712..772, U50931.1604..1749)
agctcag cctccaaagg
661 agccagcctc tccccagttc ctgaaatcct gagtgttgcc tgccagtcgc catgagaact
721 tcctaccttc tgctgtttac tctctgctta cttttgtctg agatggcctc ag
gtggtaa ctttctcaca
1621 ggccttggcc acagatctga tcattacaat tgcgtcagca gtggagggca atgtctctat
1681 tctgcctgcc cgatctttac caaaattcaa ggcacctgtt acagagggaa ggccaagtgc
1741 tgcaagtgag ctgagagtga ccagaagaaa tgacgcagaa gtgaaatgaa ctttttataa
1801 gcattctttt aataaaggaa aattgctttt gaagtat
编码蛋白MRTSYLLLFTLCLLLSEMASGGNFLTGLGHRSDHYNCVSSGGQCLYSACPIFTKIQGTCYRGKAKCCK
全长363bp 内含子:位于129-130bp间,全长6963bp
在基因组中的序列(70698-70821 63502-63735)(互补链上)
2)
RNA2258..2338,4020..4257)
CDS2281..2338,4020..4156)
ggt gaagctccca gccatcagcc
2281 atgagggtct tgtatctcct cttctcgttc ctcttcatat tcctgatgcc tcttccag
g
4021 tgtttttggt ggtataggcg atcctgttac ctgccttaag agtggagcca tatgtcatcc
4081 agtcttttgc cctagaaggt ataaacaaat tggcacctgt ggtctccctg gaacaaaatg
4141 ctgcaaaaag ccatgaggag gccaagaagc tgctgtggct gatgcggatt cagaaagggc
4201 tccctcatca gagacgtgcg acatgtaaac caaattaaac tatggtgtcc aaagata
编码蛋白:MRVLYLLFSFLFIFLMPLPGVFGGIGDPVTCLKSGAICHPVFCPRRYKQIGTCGLPGTKCCKKP
全长:319bp 内含子:位于81bp-82bp间 ,长1682bp