Preparation of cell lysates from E. coli using a French Press
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Preparation of cell lysates from E. coli using a French Press
Materials
Lysis buffer
| <center> <font>50 mM Tris-HCl pH 7.5 </font> <p> <font>50-200 mM NaCundefined</font></p> <p> <font><font>1 mM MgCl<sub>2</sub> </font> </font></p> <p> <font>5 mM DTT</font></p> <p> <font>1 mM PMSF</font></p> </center> |
