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Preparation of cell lysates from yeast using a French Press
Equipment and reagents
Lysis buffer
| <center> <font>50 mM Tris-HCl pH 7.5 </font> <p> <font>50-200 mM NaCundefined</font></p> <p> <font>1 mM EDTA, 5 mM DTT</font></p> <p> <font>1 mM PMSF</font></p> <p> <font>1 µg/ml leupeptin</font></p> <p> <font>1 µg/ml pepstatin A</font></p> </center> |
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