[分享]Real-time PCR 统计方法
丁香园论坛
4072
昨天找出了这篇关于Real-time PCR数据统计的文章，被引用了1000多次
Livak, K. J. and Schmittgen, T. D. Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2−ΔΔCT Method Methods. 25, 402, 2001.
Abstract:
The two most commonly used methods to analyze data from real-time, quantitative PCR experiments are absolute quantification and relative quantification. Absolute quantification determines the input copy number, usually by relating the PCR signal to a standard curve. Relative quantification relates the PCR signal of the target transcript in a treatment group to that of another sample such as an untreated control. The 2(-Delta Delta C(T)) method is a convenient way to analyze the relative changes in gene expression from real-time quantitative PCR experiments. The purpose of this report is to present the derivation, assumptions, and applications of the 2(-Delta Delta C(T)) method. In addition, we present the derivation and applications of two variations of the 2(-Delta Delta C(T)) method that may be useful in the analysis of real-time, quantitative PCR data.
全文地址：
http://www.sciencedirect.com/science?_ob=MImg&_imagekey=B6WN5-457MF4S-3-1&_cdi=6953&_user=554529&_orig=search&_coverDate=12%2F31%2F2001&_qd=1&_sk=999749995&view=c&wchp=dGLbVlz-zSkzS&md5=0edfc6b976b40beb08ac9a1f4bb9eb5c&ie=/sdarticle.pdf
Livak, K. J. and Schmittgen, T. D. Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2−ΔΔCT Method Methods. 25, 402, 2001.
Abstract:
The two most commonly used methods to analyze data from real-time, quantitative PCR experiments are absolute quantification and relative quantification. Absolute quantification determines the input copy number, usually by relating the PCR signal to a standard curve. Relative quantification relates the PCR signal of the target transcript in a treatment group to that of another sample such as an untreated control. The 2(-Delta Delta C(T)) method is a convenient way to analyze the relative changes in gene expression from real-time quantitative PCR experiments. The purpose of this report is to present the derivation, assumptions, and applications of the 2(-Delta Delta C(T)) method. In addition, we present the derivation and applications of two variations of the 2(-Delta Delta C(T)) method that may be useful in the analysis of real-time, quantitative PCR data.
全文地址：
http://www.sciencedirect.com/science?_ob=MImg&_imagekey=B6WN5-457MF4S-3-1&_cdi=6953&_user=554529&_orig=search&_coverDate=12%2F31%2F2001&_qd=1&_sk=999749995&view=c&wchp=dGLbVlz-zSkzS&md5=0edfc6b976b40beb08ac9a1f4bb9eb5c&ie=/sdarticle.pdf
