RIPA裂解液配方
互联网
40247
1ml RIPA buffer contains 35μl proteinase inhibitor(sigma cat no P8340) and 10μl phosphatase inhibitor
RIPA buffer 最终浓度
1M Tris-HCl pH7.5) 5 ml 50mM
NaCl 0.87g 0.15 M
Na-deoxycholale 0.1g (1%)
0.5M EDTA(pH8.0) 0.8ml
NaF 41 mg
Nonidet P40 1 ml (1%)
Aprotinin10μg/μl 100μl 10μg/ml
加水到 100ml
Aprotinin 10μg/μl ;用10mM HEPES-KOH(PH8.0)溶解,备用。