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丁香园论坛
1355
cDNA sequence: >unknown
Genomic sequence: >unknown
Blat alignment:
Length of cDNA: 5799
Start in cDNA: 0
End in cDNA: 5799
Mismatches: 0
Gaps in genomic Seq: 0 (introns included)
Gaps in cDNA: 0
Exon1_1: 966 bp,Product size 1081
Exon1_2: 966 bp,NO PRIMERS FOUND. Trying new parameters!!!
Exon1_2: 966 bp,Product size 1136
Exon1_3: 966 bp,NO PRIMERS FOUND. Trying new parameters!!!
Exon1_3: 966 bp,Product size 1131
Exon1_4: 966 bp,Product size 1125
Exon1_5: 966 bp,NO PRIMERS FOUND. Trying new parameters!!!
Exon1_5: 966 bp,Product size 1139
Exon1_6: 966 bp,Product size 1118
Exon1_1: 966 bp
OLIGO start len tm gc% any 3' rep seq
LEFT PRIMER 138 19 59.36 57.89 5.00 3.00 11.00 GAACAGGACCTCATGCAGC
RIGHT PRIMER 1218 18 60.92 66.67 3.00 2.00 12.00 GAACCCAGGAGGCAGAGC
PRODUCT SIZE: 1081, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 2.00
TARGETS (start, len~undefined: 167,993
Statistics
con too in in no tm tm high high high high
sid many tar excl bad GC too too any 3' lib poly end
ered Ns get reg GC% clamp low high compl compl sim X stab ok
Left 304 0 0 0 0 27 115 73 0 2 3 0 0 84
Right 356 0 0 0 0 20 13 259 0 10 41 0 0 13
Pair Stats:
considered 2388, unacceptable product size 2383, ok 5
primer3 release 0.9
-------------------------------------------------------------------------------------
Exon1_2: 966 bp
NO PRIMERS FOUND
Exon1_2: 966 bp
OLIGO start len tm gc% any 3' rep seq
LEFT PRIMER 1013 21 59.05 57.14 6.00 2.00 12.00 CTGCAGGACATCTAGAGAGGG
RIGHT PRIMER 2148 20 57.96 50.00 4.00 0.00 11.00 ATCCTGATCCACTTTCCTCC
PRODUCT SIZE: 1136, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 2.00
TARGETS (start, len~undefined: 1110,1016
Statistics
con too in in no tm tm high high high high
sid many tar excl bad GC too too any 3' lib poly end
ered Ns get reg GC% clamp low high compl compl sim X stab ok
Left 418 0 0 0 106 67 195 3 3 0 15 0 0 29
Right 435 0 0 0 0 58 285 13 0 0 3 0 0 76
Pair Stats:
considered 2298, unacceptable product size 2291, ok 7
primer3 release 0.9
-------------------------------------------------------------------------------------
Exon1_3: 966 bp
NO PRIMERS FOUND
Exon1_3: 966 bp
OLIGO start len tm gc% any 3' rep seq
LEFT PRIMER 2005 21 58.25 42.86 7.00 2.00 12.00 AAGGAAATGCCATCTCTTGTC
RIGHT PRIMER 3135 20 60.84 55.00 6.00 0.00 12.00 TTCTGGGCCCTCTTCTCTTC
PRODUCT SIZE: 1131, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 0.00
TARGETS (start, len~undefined: 2076,1016
Statistics
con too in in no tm tm high high high high
sid many tar excl bad GC too too any 3' lib poly end
ered Ns get reg GC% clamp low high compl compl sim X stab ok
Left 316 0 0 0 1 48 162 25 0 23 33 0 0 24
Right 386 0 0 0 0 40 173 69 0 3 8 0 0 93
Pair Stats:
considered 4412, unacceptable product size 4405, ok 7
primer3 release 0.9
-------------------------------------------------------------------------------------
Exon1_4: 966 bp
OLIGO start len tm gc% any 3' rep seq
LEFT PRIMER 2990 21 60.35 52.38 7.00 3.00 11.00 GGAGGATTGCTTGAAGCTAGG
RIGHT PRIMER 4114 20 60.79 55.00 8.00 0.00 12.00 CAGGTGGCCAGCTAGATTTG
PRODUCT SIZE: 1125, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 2.00
TARGETS (start, len~undefined: 3042,1016
Statistics
con too in in no tm tm high high high high
sid many tar excl bad GC too too any 3' lib poly end
ered Ns get reg GC% clamp low high compl compl sim X stab ok
Left 310 0 0 0 0 26 76 124 0 18 26 0 0 40
Right 254 0 0 0 3 29 70 100 0 2 0 0 0 50
Pair Stats:
considered 3347, unacceptable product size 3337, ok 10
primer3 release 0.9
-------------------------------------------------------------------------------------
Exon1_5: 966 bp
NO PRIMERS FOUND
Exon1_5: 966 bp
OLIGO start len tm gc% any 3' rep seq
LEFT PRIMER 3969 26 59.98 30.77 7.00 3.00 11.00 TTTCCCATATAATTCCAAATTCTAGC
RIGHT PRIMER 5107 21 57.68 42.86 5.00 2.00 12.00 CCTTTCACAGAAAACTGCAAG
PRODUCT SIZE: 1139, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 3.00
TARGETS (start, len~undefined: 4008,1016
Statistics
con too in in no tm tm high high high high
sid many tar excl bad GC too too any 3' lib poly end
ered Ns get reg GC% clamp low high compl compl sim X stab ok
Left 389 0 0 0 209 44 112 2 0 8 10 0 0 4
Right 324 0 0 0 69 50 172 3 1 1 7 0 0 21
Pair Stats:
considered 558, unacceptable product size 552, ok 6
primer3 release 0.9
-------------------------------------------------------------------------------------
Exon1_6: 966 bp
OLIGO start len tm gc% any 3' rep seq
LEFT PRIMER 4921 20 58.98 50.00 4.00 3.00 12.00 TTAAAGGGGAGGGATCAGTG
RIGHT PRIMER 6038 23 59.93 43.48 5.00 3.00 12.00 TTCTCCAAGGCTTTCTACAGTTG
PRODUCT SIZE: 1118, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00
TARGETS (start, len~undefined: 4977,993
Statistics
con too in in no tm tm high high high high
sid many tar excl bad GC too too any 3' lib poly end
ered Ns get reg GC% clamp low high compl compl sim X stab ok
Left 195 0 0 0 0 32 57 56 0 3 4 7 0 36
Right 207 0 0 0 0 37 129 7 0 1 3 0 0 30
Pair Stats:
considered 1468, unacceptable product size 1462, ok 6
primer3 release 0.9
Genomic sequence: >unknown
Blat alignment:
Length of cDNA: 5799
Start in cDNA: 0
End in cDNA: 5799
Mismatches: 0
Gaps in genomic Seq: 0 (introns included)
Gaps in cDNA: 0
Exon1_1: 966 bp,Product size 1081
Exon1_2: 966 bp,NO PRIMERS FOUND. Trying new parameters!!!
Exon1_2: 966 bp,Product size 1136
Exon1_3: 966 bp,NO PRIMERS FOUND. Trying new parameters!!!
Exon1_3: 966 bp,Product size 1131
Exon1_4: 966 bp,Product size 1125
Exon1_5: 966 bp,NO PRIMERS FOUND. Trying new parameters!!!
Exon1_5: 966 bp,Product size 1139
Exon1_6: 966 bp,Product size 1118
Exon1_1: 966 bp
OLIGO start len tm gc% any 3' rep seq
LEFT PRIMER 138 19 59.36 57.89 5.00 3.00 11.00 GAACAGGACCTCATGCAGC
RIGHT PRIMER 1218 18 60.92 66.67 3.00 2.00 12.00 GAACCCAGGAGGCAGAGC
PRODUCT SIZE: 1081, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 2.00
TARGETS (start, len~undefined: 167,993
Statistics
con too in in no tm tm high high high high
sid many tar excl bad GC too too any 3' lib poly end
ered Ns get reg GC% clamp low high compl compl sim X stab ok
Left 304 0 0 0 0 27 115 73 0 2 3 0 0 84
Right 356 0 0 0 0 20 13 259 0 10 41 0 0 13
Pair Stats:
considered 2388, unacceptable product size 2383, ok 5
primer3 release 0.9
-------------------------------------------------------------------------------------
Exon1_2: 966 bp
NO PRIMERS FOUND
Exon1_2: 966 bp
OLIGO start len tm gc% any 3' rep seq
LEFT PRIMER 1013 21 59.05 57.14 6.00 2.00 12.00 CTGCAGGACATCTAGAGAGGG
RIGHT PRIMER 2148 20 57.96 50.00 4.00 0.00 11.00 ATCCTGATCCACTTTCCTCC
PRODUCT SIZE: 1136, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 2.00
TARGETS (start, len~undefined: 1110,1016
Statistics
con too in in no tm tm high high high high
sid many tar excl bad GC too too any 3' lib poly end
ered Ns get reg GC% clamp low high compl compl sim X stab ok
Left 418 0 0 0 106 67 195 3 3 0 15 0 0 29
Right 435 0 0 0 0 58 285 13 0 0 3 0 0 76
Pair Stats:
considered 2298, unacceptable product size 2291, ok 7
primer3 release 0.9
-------------------------------------------------------------------------------------
Exon1_3: 966 bp
NO PRIMERS FOUND
Exon1_3: 966 bp
OLIGO start len tm gc% any 3' rep seq
LEFT PRIMER 2005 21 58.25 42.86 7.00 2.00 12.00 AAGGAAATGCCATCTCTTGTC
RIGHT PRIMER 3135 20 60.84 55.00 6.00 0.00 12.00 TTCTGGGCCCTCTTCTCTTC
PRODUCT SIZE: 1131, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 0.00
TARGETS (start, len~undefined: 2076,1016
Statistics
con too in in no tm tm high high high high
sid many tar excl bad GC too too any 3' lib poly end
ered Ns get reg GC% clamp low high compl compl sim X stab ok
Left 316 0 0 0 1 48 162 25 0 23 33 0 0 24
Right 386 0 0 0 0 40 173 69 0 3 8 0 0 93
Pair Stats:
considered 4412, unacceptable product size 4405, ok 7
primer3 release 0.9
-------------------------------------------------------------------------------------
Exon1_4: 966 bp
OLIGO start len tm gc% any 3' rep seq
LEFT PRIMER 2990 21 60.35 52.38 7.00 3.00 11.00 GGAGGATTGCTTGAAGCTAGG
RIGHT PRIMER 4114 20 60.79 55.00 8.00 0.00 12.00 CAGGTGGCCAGCTAGATTTG
PRODUCT SIZE: 1125, PAIR ANY COMPL: 5.00, PAIR 3' COMPL: 2.00
TARGETS (start, len~undefined: 3042,1016
Statistics
con too in in no tm tm high high high high
sid many tar excl bad GC too too any 3' lib poly end
ered Ns get reg GC% clamp low high compl compl sim X stab ok
Left 310 0 0 0 0 26 76 124 0 18 26 0 0 40
Right 254 0 0 0 3 29 70 100 0 2 0 0 0 50
Pair Stats:
considered 3347, unacceptable product size 3337, ok 10
primer3 release 0.9
-------------------------------------------------------------------------------------
Exon1_5: 966 bp
NO PRIMERS FOUND
Exon1_5: 966 bp
OLIGO start len tm gc% any 3' rep seq
LEFT PRIMER 3969 26 59.98 30.77 7.00 3.00 11.00 TTTCCCATATAATTCCAAATTCTAGC
RIGHT PRIMER 5107 21 57.68 42.86 5.00 2.00 12.00 CCTTTCACAGAAAACTGCAAG
PRODUCT SIZE: 1139, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 3.00
TARGETS (start, len~undefined: 4008,1016
Statistics
con too in in no tm tm high high high high
sid many tar excl bad GC too too any 3' lib poly end
ered Ns get reg GC% clamp low high compl compl sim X stab ok
Left 389 0 0 0 209 44 112 2 0 8 10 0 0 4
Right 324 0 0 0 69 50 172 3 1 1 7 0 0 21
Pair Stats:
considered 558, unacceptable product size 552, ok 6
primer3 release 0.9
-------------------------------------------------------------------------------------
Exon1_6: 966 bp
OLIGO start len tm gc% any 3' rep seq
LEFT PRIMER 4921 20 58.98 50.00 4.00 3.00 12.00 TTAAAGGGGAGGGATCAGTG
RIGHT PRIMER 6038 23 59.93 43.48 5.00 3.00 12.00 TTCTCCAAGGCTTTCTACAGTTG
PRODUCT SIZE: 1118, PAIR ANY COMPL: 4.00, PAIR 3' COMPL: 1.00
TARGETS (start, len~undefined: 4977,993
Statistics
con too in in no tm tm high high high high
sid many tar excl bad GC too too any 3' lib poly end
ered Ns get reg GC% clamp low high compl compl sim X stab ok
Left 195 0 0 0 0 32 57 56 0 3 4 7 0 36
Right 207 0 0 0 0 37 129 7 0 1 3 0 0 30
Pair Stats:
considered 1468, unacceptable product size 1462, ok 6
primer3 release 0.9
