紧急求救引物设计，请高手们帮我看看

我现在急需做试验，但是我的引物还没有设计出来，所以万分着急。我想做XIAP 的RT-PCR，我现在找到它的mRNA序列为
1 gaaaaggtgg acaagtccta ttttcaagag aagatgactt ttaacagttt tgaaggatct
61 aaaacttgtg tacctgcaga catcaataag gaagaagaat ttgtagaaga gtttaataga
121 ttaaaaactt ttgctaattt tccaagtggt agtcctgttt cagcatcaac actggcacga
181 gcagggtttc tttatactgg tgaaggagat accgtgcggt gctttagttg tcatgcagct
241 gtagatagat ggcaatatgg agactcagca gttggaagac acaggaaagt atccccaaat
301 tgcagattta tcaacggctt ttatcttgaa aatagtgcca cgcagtctac aaattctggt
361 atccagaatg gtcagtacaa agttgaaaac tatctgggaa gcagagatca ttttgcctta
421 gacaggccat ctgagacaca tgcagactat cttttgagaa ctgggcaggt tgtagatata
481 tcagacacca tatacccgag gaaccctgcc atgtattgtg aagaagctag attaaagtcc
541 tttcagaact ggccagacta tgctcaccta accccaagag agttagcaag tgctggactc
601 tactacacag gtattggtga ccaagtgcag tgcttttgtt gtggtggaaa actgaaaaat
661 tgggaacctt gtgatcgtgc ctggtcagaa cacaggcgac actttcctaa ttgcttcttt
721 gttttgggcc ggaatcttaa tattcgaagt gaatctgatg ctgtgagttc tgataggaat
781 ttcccaaatt caacaaatct tccaagaaat ccatccatgg cagattatga agcacggatc
841 tttacttttg ggacatggat atactcagtt aacaaggagc agcttgcaag agctggattt
901 tatgctttag gtgaaggtga taaagtaaag tgctttcact gtggaggagg gctaactgat
961 tggaagccca gtgaagaccc ttgggaacaa catgctaaat ggtatccagg gtgcaaatat
1021 ctgttagaac agaagggaca agaatatata aacaatattc atttaactca ttcacttgag
1081 gagtgtctgg taagaactac tgagaaaaca ccatcactaa ctagaagaat tgatgatacc
1141 atcttccaaa atcctatggt acaagaagct atacgaatgg ggttcagttt caaggacatt
1201 aagaaaataa tggaggaaaa aattcagata tctgggagca actataaatc acttgaggtt
1261 ctggttgcag atctagtgaa tgctcagaaa gacagtatgc aagatgagtc aagtcagact
1321 tcattacaga aagagattag tactgaagag cagctaaggc gcctgcaaga ggagaagctt
1381 tgcaaaatct gtatggatag aaatattgct atcgtttttg ttccttgtgg acatctagtc
1441 acttgtaaac aatgtgctga agcagttgac aagtgtccca tgtgctacac agtcattact
1501 ttcaagcaaa aaatttttat gtcttaatct aactctatag taggcatgtt atgttgttct
1561 tattaccctg attgaatgtg tgatgtgaac tgactttaag taatcaggat tgaattccat
1621 tagcatttgc taccaagtag gaaaaaaaat gtacatggca gtgttttagt tggcaatata
1681 atctttgaat ttcttgattt ttcagggtat tagctgtatt atccattttt tttactgtta
1741 tttaattgaa accatagact aagaataaga agcatcatac tataactgaa cacaatgtgt
1801 attcatagta tactgattta atttctaagt gtaagtgaat taatcatctg gattttttat
1861 tcttttcaga taggcttaac aaatggagct ttctgtatat aaatgtggag attagagtta
1921 atctccccaa tcacataatt tgttttgtgt gaaaaaggaa taaattgttc catgctggtg
1981 gaaagataga gattgttttt agaggttggt tgttgtgttt taggattctg tccattttct
2041 tgtaaaggga taaacacgga cgtgtgcgaa atatgtttgt aaagtgattt gccattgttg
2101 aaagcgtatt taatgataga atactatcga gccaacatgt actgacatgg aaagatgtca
2161 gagatatgtt aagtgtaaaa tgcaagtggc gggacactat gtatagtctg agccagatca
2221 aagtatgtat gttgttaata tgcatagaac gagagatttg gaaagatata caccaaactg
2281 ttaaatgtgg tttctcttcg gggagggggg gattggggga ggggccccag aggggtttta
2341 gaggggcctt ttcactttcg acttttttca ttttgttctg ttcggatttt ttataagtat
2401 gtagaccccg aagggtttta tgggaactaa catcagtaac ctaacccccg tgactatcct
2461 gtgctcttcc tagggagctg tgttgtttcc cacccaccac ccttccctct gaacaaatgc
2521 ctgagtgctg gggcactttg
我用Primer3设计出5对引物：
1 OLIGO start len tm gc% any 3' seq
LEFT PRIMER 1021 20 59.94 50.00 4.00 0.00 gaagacccttgggaacaaca
RIGHT PRIMER 1258 20 59.94 50.00 3.00 0.00 tgtccttgaaactgaacccc
2 LEFT PRIMER 1020 20 59.94 50.00 4.00 0.00 tgaagacccttgggaacaac
RIGHT PRIMER 1258 20 59.94 50.00 3.00 0.00 tgtccttgaaactgaacccc
3 LEFT PRIMER 1021 20 59.94 50.00 4.00 0.00 gaagacccttgggaacaaca
RIGHT PRIMER 1257 20 59.94 50.00 3.00 0.00 gtccttgaaactgaacccca
4 LEFT PRIMER 1020 20 59.94 50.00 4.00 0.00 tgaagacccttgggaacaac
RIGHT PRIMER 1257 20 59.94 50.00 3.00 0.00 gtccttgaaactgaacccca
5 LEFT PRIMER 1020 20 59.94 50.00 4.00 0.00 tgaagacccttgggaacaac
RIGHT PRIMER 1257 20 59.94 50.00 3.00 0.00 gtccttgaaactgaacccca
因为我刚开始做试验，虽然也看一了一些关于引物设计的资料，但是觉得还是不太会，请大家帮我看看哪个引物比较好？因为我的实验很紧急，麻烦大家了。在此先谢过了。