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help:请各位高手帮忙看看我设计的SD大鼠caspase-3的引物

丁香园论坛

2513
我最近用一在线的引物设计软件设计了一对SD大鼠caspase-3 的引物,不知行不,请高手多多帮忙.
结果如下:
Primer3 Output

--------------------------------------------------------------------------------

WARNING: Numbers in input sequence were deleted.

No mispriming library specified
Using 1-based sequence positions
OLIGO start len tm gc% any 3' seq
LEFT PRIMER 1342 20 58.96 50.00 3.00 0.00 gaatccacgagcagagtcaa
RIGHT PRIMER 1557 20 60.25 50.00 3.00 0.00 tagccttcaacaagccaacc
SEQUENCE SIZE: 2484
INCLUDED REGION SIZE: 2484

PRODUCT SIZE: 216, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 0.00

1 gggatcaaagcttagtgtcctgaggtgcggagcttggaacgcgaagaaaagtgaccatgg

61 acaacaacgaaacctccgtggattcaaaatccattaataattttgaaacaaagactatcc

121 atggaagcaagtcgatggactctggaatatatctggacagcagttacaaaatggattacc

181 ctgaaatgggcttgtgtataataattaataataagaacttccataaaagcactggaatgt

241 cagctcgcaatggtaccgatgtcgatgcagctaacctcagagagacattcatggccctga

301 aatacgaagtcaggaataaaaatgaccttactcgtgaagaaattatggaattgatggata

361 gtgtttctaaggaagatcacagcaaaaggagcagttttgtgtgtgtgattctaagtcatg

421 gagatgaaggagtaatttttggaacgaacggacctgtggacctgaaaaaactaactagtt

481 tcttcagaggcgactactgccggagtctgactggaaagccgaaactcttcatcattcagg

541 cctgccgaggtacagagctggactgcggtattgagacagacagtggaactgacgatgata

601 tggcatgccagaagataccagtggaggccgacttcctgtatgcttactctaccgcacccg

661 gttactattcctggagaaattcaagggacgggtcatggttcatccagtcactttgcgcca

721 tgctgaaactgtacgcgcacaagctggaattcatgcacatcctcactcgtgttaaccgga

781 aggtggccacggaatttgagtccttctccctggacgccactttccatgcaaagaaacaga

841 tcccgtgtattgtgtcaatgctcacaaaagaactgtacttttatcactaaaggaatgact

901 gggagtggggtaggggcatgtttctgttttggtttttttttttggtttttgttttttgtt

961 tttttttttttatttgaatgccaaatgagaaaactgtcagggagactttttttttcccct

1021 ctcatttaaatcaaatccgatgttccaggtcgtcattgaacaataccactgcctgcaatg

1081 cagccacaatacaatacctcagctttgatatcaggtagttgaaatgaaatttaattagga

1141 acaaataaatgtcaataatggtacaaccatcatagaagaaattggaaagtcacactttgg

1201 taattagagagatttggtgccactatgaatttgaaattacgaagcagtgatgatcttata

1261 ccccctcctcacatctagaatgtcactttagttttcgttaagcataggaacgataatgtc

1321 aagtggtatgtcttagaacttgaatccacgagcagagtcaaaggctggttttggaactga
>>>>>>>>>>>>>>>>>>>>

1381 tctgtggaaatgaagagttggagcactgtagcacacattatagctactggttttgtgaca

1441 gttgaccaggtcacatatttattatttaagacaaaagtcgtgttttattattggaacaaa

1501 aataactttatatgagaggaaaaccatgtgtgaacttggttggcttgttgaaggctacca
<<<<<<<<<<<<<<<<<<<<

1561 tccttcagtggtggacatgacgacagggtgctacgatccaccagcatttgtttcttactt

1621 attcttatgcaaatcagcctaattttacagaccagcaaaatatctaaagagcgattatgc

1681 agcagcctcaaattgttgactattagaatggaataaaggaattctacaggaacagaccat

1741 aataccagctgtcagtcagagcgtaaggaaaggagaggtggcatctcctgtgattggaga

1801 gaagatggtttgagccggagcagagccatgggcacgtcttcagaaaaggcctgctagttg

1861 gacccaccttgtgagcactggtctgtgtggatgatgtccaagttaccacatgagactgaa

1921 ccagatcagaagctcctgcaaaaaggctttcccagagtgctgagcatctcaagaaagttc

1981 actggactccatgtacagagtctgtagcatgctgacttttctgttgaagtttacaatgaa

2041 aggaaaatgatggtgatatttgtaccgtcactagaaatgaagggccctggcacacgggac

2101 ttggaaagcatccagcaataggccctgcagcccatgtggaggagctgcagactggagtcc

2161 acccctcctccccatcagggcgtctgtgctccaggcttccttaatcatggaggtgctgag

2221 tcatcgctggctctccctgtgggtccagcatttcccataagcctccttattgcacaaaga

2281 aaggaaggaactcacattataactgatagtaggttcttgtttagactggatttactgtta

2341 actcttttctaagtaaatacagcatgtggtggtattttaaaatgtgttcccatgtgacaa

2401 ttttataaaagtcagatatcttgcatttttatttcaaaacataaattcagatttaaaatt

2461 aaaaaaaaaaaaaaaaaaaaaaaa

KEYS (in order of precedence):
>>>>>> left primer
<<<<<< right primer

ADDITIONAL OLIGOS
start len tm gc% any 3' seq

1 LEFT PRIMER 1342 20 58.96 50.00 3.00 0.00 gaatccacgagcagagtcaa
RIGHT PRIMER 1550 20 59.73 45.00 3.00 1.00 caacaagccaaccaagttca
PRODUCT SIZE: 209, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 2.00

2 LEFT PRIMER 1342 20 58.96 50.00 3.00 0.00 gaatccacgagcagagtcaa
RIGHT PRIMER 1554 20 60.66 50.00 3.00 0.00 ccttcaacaagccaaccaag
PRODUCT SIZE: 213, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 0.00

3 LEFT PRIMER 1342 20 58.96 50.00 3.00 0.00 gaatccacgagcagagtcaa
RIGHT PRIMER 1546 20 59.19 45.00 3.00 0.00 aagccaaccaagttcacaca
PRODUCT SIZE: 205, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 2.00

4 LEFT PRIMER 1342 20 58.96 50.00 3.00 0.00 gaatccacgagcagagtcaa
RIGHT PRIMER 1548 20 59.19 45.00 3.00 0.00 acaagccaaccaagttcaca
PRODUCT SIZE: 207, PAIR ANY COMPL: 3.00, PAIR 3' COMPL: 2.00

Statistics
con too in in no tm tm high high high
sid many tar excl bad GC too too any 3' poly end
ered Ns get reg GC% clamp low high compl compl X stab ok
Left 9195 0 0 0 698 0 2454 2424 693 0 1719 92 1115
Right 9393 0 0 0 1124 0 2439 2270 32 0 1692 88 1748
Pair Stats:
considered 61917, unacceptable product size 61821, high any compl 88, ok 8
primer3 release 1.0

(primer3_www_results.cgi v 0.4)
有同学说,有个引物评分,请问师兄我这对引物有多少分?谢谢!!!!!!
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