Epitope Mapping Using Phage-Display Random Fragment Libraries

Phage-display has become a method of choice for epitope mapping and has been successfully used in numerous published studies. Although the inaugural studies were all done with random peptide libraries (see Chapter “Epitope Mapping Using Phage Display Peptide Libraries”), gene- or genome-targeted random fragment libraries have proven to be a more effective epitope mapping approach for some antibodies. In this chapter, we describe the mapping of linear and conformational epitopes of the major African swine fever virus capsid protein using monoclonal as well as polyclonal antibodies.