Epitope Mapping Using Random Fragment Expression Libraries in Phages

The efficient detection of antigenic epitopes in a protein by screening a recombinant λ phage expression sublibrary of the antigen cDNA was first described by Mehra et al. (1 ). Subsequently, the method has found multiple applications. We have used it to identify the epitopes for a panel of monoclonal antibodies (MAbs) raised against the canine myocardial Na⁺ -Ca²⁺ exchanger (2 ), which was first cloned by Nicoll et al. (3 ).