请教:如何找几个亚型基因的保守序列?
丁香园论坛
2747
我想根据keratin基因(有30多个亚型)的共有保守序列,设计引物扩增,那位大侠能帮我找一下好吗?谢谢!
keratin 5
atgtctc gccagtcaag
181 tgtgtccttc cggagcgggg gcagtcgtag cttcagcacc gcctctgcca tcaccccgtc
241 tgtctcccgc accagcttca cctccgtgtc ccggtccggg ggtggcggtg gtggtggctt
301 cggcagggtc agccttgcgg gtgcttgtgg agtgggtggc tatggcagcc ggagcctcta
361 caacctgggg ggctccaaga ggatatccat cagcactaga ggaggcagct tcaggaaccg
421 gtttggtgct ggtgctggag gcggctatgg ctttggaggt ggtgccggta gtggatttgg
481 tttcggcggt ggagctggtg gtggctttgg gctcggtggc ggagctggct ttggaggtgg
541 cttcggtggc cctggctttc ctgtctgccc tcctggaggt atccaagagg tcactgtcaa
601 ccagagtctc ctgactcccc tcaacctgca aatcgacccc agcatccaga gggtgaggac
661 cgaggagcgc gagcagatca agaccctcaa caataagttt gcctccttca tcgacaaggt
721 gcggttcctg gagcagcaga acaaggttct ggacaccaag tggaccctgc tgcaggagca
781 gggcaccaag actgtgaggc agaacctgga gccgttgttc gagcagtaca tcaacaacct
841 caggaggcag ctggacagca tcgtggggga acggggccgc ctggactcag agctgagaaa
901 catgcaggac ctggtggaag acttcaagaa caagtatgag gatgaaatca acaagcgtac
961 cactgctgag aatgagtttg tgatgctgaa gaaggatgta gatgctgcct acatgaacaa
1021 ggtggagctg gaggccaagg ttgatgcact gatggatgag attaacttca tgaagatgtt
1081 ctttgatgcg gagctgtccc agatgcagac gcatgtctct gacacctcag tggtcctctc
1141 catggacaac aaccgcaacc tggacctgga tagcatcatc gctgaggtca aggcccagta
1201 tgaggagatt gccaaccgca gccggacaga agccgagtcc tggtatcaga ccaagtatga
1261 ggagctgcag cagacagctg gccggcatgg cgatgacctc cgcaacacca agcatgagat
1321 cacagagatg aaccggatga tccagaggct gagagccgag attgacaatg tcaagaaaca
1381 gtgcgccaat ctgcagaacg ccattgcgga tgccgagcag cgtggggagc tggccctcaa
1441 ggatgccagg aacaagctgg ccgagctgga ggaggccctg cagaaggcca agcaggacat
1501 ggcccggctg ctgcgtgagt accaggagct catgaacacc aagctggccc tggacgtgga
1561 gatcgccact taccgcaagc tgctggaggg cgaggaatgc agactcagtg gagaaggagt
1621 tggaccagtc aacatctctg ttgtcacaag cagtgtttcc tctggatatg gcagtggcag
1681 tggctatggc ggtggcctcg gtggaggtct tggcggcggc ctcggtggag gtcttgccgg
1741 aggtagcagt ggaagctact actccagcag cagtgggggt gtcggcctag gtggtgggct
1801 cagtgtgggg ggctctggct tcagtgcaag cagtggccga gggctggggg tgggctttgg
1861 cagtggcggg ggtagcagct ccagcgtcaa atttgtctcc accacctcct cctcccggaa
1921 gagcttcaag agctaa
keratin 8
a
61 tgtccatcag ggtgacccag aagtcctaca aggtgtccac ctctggcccc cgggccttca
121 gcagccgctc ctacacgagt gggcccggtt cccgcatcag ctcctcgagc ttctcccgag
181 tgggcagcag caactttcgc ggtggcctgg gcggcggcta tggtggggcc agcggcatgg
241 gaggcatcac cgcagttacg gtcaaccaga gcctgctgag cccccttgtc ctggaggtgg
301 accccaacat ccaggccgtg cgcacccagg agaaggagca gatcaagacc ctcaacaaca
361 agtttgcctc cttcatagac aaggtacggt tcctggagca gcagaacaag atgctggaga
421 ccaagtggag cctcctgcag cagcagaaga cggctcgaag caacatggac aacatgttcg
481 agagctacat caacaacctt aggcggcagc tggagactct gggccaggag aagctgaagc
541 tggaggcgga gcttggcaac atgcaggggc tggtggagga cttcaagaac aagtatgagg
601 atgagatcaa taagcgtaca gagatggaga acgaatttgt cctcatcaag aaggatgtgg
661 atgaagctta catgaacaag gtagagctgg agtctcgcct ggaagggctg accgacgaga
721 tcaacttcct caggcagcta tatgaagagg agatccggga gctgcagtcc cagatctcgg
781 acacatctgt ggtgctgtcc atggacaaca gccgctccct ggacatggac agcatcattg
841 ctgaggtcaa ggcacagtac gaggatattg ccaaccgcag ccgggctgag gctgagagca
901 tgtaccagat caagtatgag gagctgcaga gcctggctgg gaagcacggg gatgacctgc
961 ggcgcacaaa gactgagatc tctgagatga accggaacat cagccggctc caggctgaga
1021 ttgagggcct caaaggccag agggcttccc tggaggccgc cattgcagat gccgagcagc
1081 gtggagagct ggccattaag gatgccaacg ccaagttgtc cgagctggag gccgccctgc
1141 agcgggccaa gcaggacatg gcgcggcagc tgcgtgagta ccaggagctg atgaacgtca
1201 agctggccct ggacatcgag atcgccacct acaggaagct gctggagggc gaggagagcc
1261 ggctggagtc tgggatgcag aacatgagta ttcatacgaa gaccaccagc ggctatgcag
1321 gtggtctgag ctcggcctat gggggcctca caagccccgg cctcagctac agcctgggct
1381 ccagctttgg ctctggcgcg ggctccagct ccttcagccg caccagctcc tccagggccg
1441 tggttgtgaa gaagatcgag acacgtgatg ggaagctggt gtctgagtcc tctgacgtcc
1501 tgcccaagtg a
keratin 18
atga gcttcaccac
61 tcgctccacc ttctccacca actaccggtc cctgggctct gtccaggcgc ccagctacgg
121 cgcccggccg gtcagcagcg cggccagcgt ctatgcaggc gctgggggct ctggttcccg
181 gatctccgtg tcccgctcca ccagcttcag gggcggcatg gggtccgggg gcctggccac
241 cgggatagcc gggggtctgg caggaatggg aggcatccag aacgagaagg agaccatgca
301 aagcctgaac gaccgcctgg cctcttacct ggacagagtg aggagcctgg agaccgagaa
361 ccggaggctg gagagcaaaa tccgggagca cttggagaag aagggacccc aggtcagaga
421 ctggagccat tacttcaaga tcatcgagga cctgagggct cagatcttcg caaatactgt
481 ggacaatgcc cgcatcgttc tgcagattga caatgcccgt cttgctgctg atgactttag
541 agtcaagtat gagacagagc tggccatgcg ccagtctgtg gagaacgaca tccatgggct
601 ccgcaaggtc attgatgaca ccaatatcac acgactgcag ctggagacag agatcgaggc
661 tctcaaggag gagctgctct tcatgaagaa gaaccacgaa gaggaagtaa aaggcctaca
721 agcccagatt gccagctctg ggttgaccgt ggaggtagat gcccccaaat ctcaggacct
781 cgccaagatc atggcagaca tccgggccca atatgacgag ctggctcgga agaaccgaga
841 ggagctagac aagtactggt ctcagcagat tgaggagagc accacagtgg tcaccacaca
901 gtctgctgag gttggagctg ctgagacgac gctcacagag ctgagacgta cagtccagtc
961 cttggagatc gacctggact ccatgagaaa tctgaaggcc agcttggaga acagcctgag
1021 ggaggtggag gcccgctacg ccctacagat ggagcagctc aacgggatcc tgctgcacct
1081 tgagtcagag ctggcacaga cccgggcaga gggacagcgc caggcccagg agtatgaggc
1141 cctgctgaac atcaaggtca agctggaggc tgagatcgcc acctaccgcc gcctgctgga
1201 agatggcgag gactttaatc ttggtgatgc cttggacagc agcaactcca tgcaaaccat
1261 ccaaaagacc accacccgcc ggatagtgga tggcaaagtg gtgtctgaga ccaatgacac
1321 caaagttctg aggcattaa
keratin 5
atgtctc gccagtcaag
181 tgtgtccttc cggagcgggg gcagtcgtag cttcagcacc gcctctgcca tcaccccgtc
241 tgtctcccgc accagcttca cctccgtgtc ccggtccggg ggtggcggtg gtggtggctt
301 cggcagggtc agccttgcgg gtgcttgtgg agtgggtggc tatggcagcc ggagcctcta
361 caacctgggg ggctccaaga ggatatccat cagcactaga ggaggcagct tcaggaaccg
421 gtttggtgct ggtgctggag gcggctatgg ctttggaggt ggtgccggta gtggatttgg
481 tttcggcggt ggagctggtg gtggctttgg gctcggtggc ggagctggct ttggaggtgg
541 cttcggtggc cctggctttc ctgtctgccc tcctggaggt atccaagagg tcactgtcaa
601 ccagagtctc ctgactcccc tcaacctgca aatcgacccc agcatccaga gggtgaggac
661 cgaggagcgc gagcagatca agaccctcaa caataagttt gcctccttca tcgacaaggt
721 gcggttcctg gagcagcaga acaaggttct ggacaccaag tggaccctgc tgcaggagca
781 gggcaccaag actgtgaggc agaacctgga gccgttgttc gagcagtaca tcaacaacct
841 caggaggcag ctggacagca tcgtggggga acggggccgc ctggactcag agctgagaaa
901 catgcaggac ctggtggaag acttcaagaa caagtatgag gatgaaatca acaagcgtac
961 cactgctgag aatgagtttg tgatgctgaa gaaggatgta gatgctgcct acatgaacaa
1021 ggtggagctg gaggccaagg ttgatgcact gatggatgag attaacttca tgaagatgtt
1081 ctttgatgcg gagctgtccc agatgcagac gcatgtctct gacacctcag tggtcctctc
1141 catggacaac aaccgcaacc tggacctgga tagcatcatc gctgaggtca aggcccagta
1201 tgaggagatt gccaaccgca gccggacaga agccgagtcc tggtatcaga ccaagtatga
1261 ggagctgcag cagacagctg gccggcatgg cgatgacctc cgcaacacca agcatgagat
1321 cacagagatg aaccggatga tccagaggct gagagccgag attgacaatg tcaagaaaca
1381 gtgcgccaat ctgcagaacg ccattgcgga tgccgagcag cgtggggagc tggccctcaa
1441 ggatgccagg aacaagctgg ccgagctgga ggaggccctg cagaaggcca agcaggacat
1501 ggcccggctg ctgcgtgagt accaggagct catgaacacc aagctggccc tggacgtgga
1561 gatcgccact taccgcaagc tgctggaggg cgaggaatgc agactcagtg gagaaggagt
1621 tggaccagtc aacatctctg ttgtcacaag cagtgtttcc tctggatatg gcagtggcag
1681 tggctatggc ggtggcctcg gtggaggtct tggcggcggc ctcggtggag gtcttgccgg
1741 aggtagcagt ggaagctact actccagcag cagtgggggt gtcggcctag gtggtgggct
1801 cagtgtgggg ggctctggct tcagtgcaag cagtggccga gggctggggg tgggctttgg
1861 cagtggcggg ggtagcagct ccagcgtcaa atttgtctcc accacctcct cctcccggaa
1921 gagcttcaag agctaa
keratin 8
a
61 tgtccatcag ggtgacccag aagtcctaca aggtgtccac ctctggcccc cgggccttca
121 gcagccgctc ctacacgagt gggcccggtt cccgcatcag ctcctcgagc ttctcccgag
181 tgggcagcag caactttcgc ggtggcctgg gcggcggcta tggtggggcc agcggcatgg
241 gaggcatcac cgcagttacg gtcaaccaga gcctgctgag cccccttgtc ctggaggtgg
301 accccaacat ccaggccgtg cgcacccagg agaaggagca gatcaagacc ctcaacaaca
361 agtttgcctc cttcatagac aaggtacggt tcctggagca gcagaacaag atgctggaga
421 ccaagtggag cctcctgcag cagcagaaga cggctcgaag caacatggac aacatgttcg
481 agagctacat caacaacctt aggcggcagc tggagactct gggccaggag aagctgaagc
541 tggaggcgga gcttggcaac atgcaggggc tggtggagga cttcaagaac aagtatgagg
601 atgagatcaa taagcgtaca gagatggaga acgaatttgt cctcatcaag aaggatgtgg
661 atgaagctta catgaacaag gtagagctgg agtctcgcct ggaagggctg accgacgaga
721 tcaacttcct caggcagcta tatgaagagg agatccggga gctgcagtcc cagatctcgg
781 acacatctgt ggtgctgtcc atggacaaca gccgctccct ggacatggac agcatcattg
841 ctgaggtcaa ggcacagtac gaggatattg ccaaccgcag ccgggctgag gctgagagca
901 tgtaccagat caagtatgag gagctgcaga gcctggctgg gaagcacggg gatgacctgc
961 ggcgcacaaa gactgagatc tctgagatga accggaacat cagccggctc caggctgaga
1021 ttgagggcct caaaggccag agggcttccc tggaggccgc cattgcagat gccgagcagc
1081 gtggagagct ggccattaag gatgccaacg ccaagttgtc cgagctggag gccgccctgc
1141 agcgggccaa gcaggacatg gcgcggcagc tgcgtgagta ccaggagctg atgaacgtca
1201 agctggccct ggacatcgag atcgccacct acaggaagct gctggagggc gaggagagcc
1261 ggctggagtc tgggatgcag aacatgagta ttcatacgaa gaccaccagc ggctatgcag
1321 gtggtctgag ctcggcctat gggggcctca caagccccgg cctcagctac agcctgggct
1381 ccagctttgg ctctggcgcg ggctccagct ccttcagccg caccagctcc tccagggccg
1441 tggttgtgaa gaagatcgag acacgtgatg ggaagctggt gtctgagtcc tctgacgtcc
1501 tgcccaagtg a
keratin 18
atga gcttcaccac
61 tcgctccacc ttctccacca actaccggtc cctgggctct gtccaggcgc ccagctacgg
121 cgcccggccg gtcagcagcg cggccagcgt ctatgcaggc gctgggggct ctggttcccg
181 gatctccgtg tcccgctcca ccagcttcag gggcggcatg gggtccgggg gcctggccac
241 cgggatagcc gggggtctgg caggaatggg aggcatccag aacgagaagg agaccatgca
301 aagcctgaac gaccgcctgg cctcttacct ggacagagtg aggagcctgg agaccgagaa
361 ccggaggctg gagagcaaaa tccgggagca cttggagaag aagggacccc aggtcagaga
421 ctggagccat tacttcaaga tcatcgagga cctgagggct cagatcttcg caaatactgt
481 ggacaatgcc cgcatcgttc tgcagattga caatgcccgt cttgctgctg atgactttag
541 agtcaagtat gagacagagc tggccatgcg ccagtctgtg gagaacgaca tccatgggct
601 ccgcaaggtc attgatgaca ccaatatcac acgactgcag ctggagacag agatcgaggc
661 tctcaaggag gagctgctct tcatgaagaa gaaccacgaa gaggaagtaa aaggcctaca
721 agcccagatt gccagctctg ggttgaccgt ggaggtagat gcccccaaat ctcaggacct
781 cgccaagatc atggcagaca tccgggccca atatgacgag ctggctcgga agaaccgaga
841 ggagctagac aagtactggt ctcagcagat tgaggagagc accacagtgg tcaccacaca
901 gtctgctgag gttggagctg ctgagacgac gctcacagag ctgagacgta cagtccagtc
961 cttggagatc gacctggact ccatgagaaa tctgaaggcc agcttggaga acagcctgag
1021 ggaggtggag gcccgctacg ccctacagat ggagcagctc aacgggatcc tgctgcacct
1081 tgagtcagag ctggcacaga cccgggcaga gggacagcgc caggcccagg agtatgaggc
1141 cctgctgaac atcaaggtca agctggaggc tgagatcgcc acctaccgcc gcctgctgga
1201 agatggcgag gactttaatc ttggtgatgc cttggacagc agcaactcca tgcaaaccat
1261 ccaaaagacc accacccgcc ggatagtgga tggcaaagtg gtgtctgaga ccaatgacac
1321 caaagttctg aggcattaa