【旧贴整理】重组PCR
丁香园论坛
1.重组PCR原理
http://www.dxy.cn/bbs/actions/archive/post/1263309_0.html
2.是直接以初次PCR扩增产物作重叠延伸，还是电泳后切胶回收后再做
http://www.dxy.cn/bbs/actions/archive/post/5540543_0.html
http://www.dxy.cn/bbs/thread/4745715
http://www.dxy.cn/bbs/post/view?bid=67&id=617346&sty=3&keywords=SOE
http://www.dxy.cn/bbs/thread/4200891
http://www.dxy.cn/bbs/post/view?bid=67&id=5131656&sty=3&keywords=SOE
http://www.dxy.cn/bbs/actions/archive/post/1589444_1.html
http://www.dxy.cn/bbs/actions/archive/post/5542516_0.html
3.选用高保真DNA酶
http://www.dxy.cn/bbs/actions/archive/post/5540543_0.html
4,退火温度设计
http://www.dxy.cn/bbs/actions/archive/post/334090_0.html
5.退火温度过高
http://www.dxy.cn/bbs/actions/archive/post/6302006_0.html
6.模版处理
http://www.dxy.cn/bbs/actions/archive/post/875796_0.html
7.重组PCR应用
http://www.dxy.cn/bbs/post/view?bid=64&id=2869734&sty=3&keywords=%D6%D8%D7%E9PCR
http://www.dxy.cn/bbs/post/view?bid=67&id=1607534&sty=3&keywords=%D6%D8%D7%E9PCR
http://www.dxy.cn/bbs/post/view?bid=64&id=4802969&sty=3&keywords=%D6%D8%D7%E9PCR
8.SOE pcr
http://www.dxy.cn/bbs/post/view?bid=67&id=1240902&sty=3&keywords=SOE
9.soe引物设计
http://www.dxy.cn/bbs/post/view?bid=67&id=5991031&sty=3&keywords=SOE
10。有关两步法PCR和SOE法的基础问题
http://www.dxy.cn/bbs/actions/archive/post/1596165_0.html
11重叠延伸PCR
http://www.dxy.cn/bbs/actions/archive/post/3857682_0.html
