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海洋微生物的培养基:Marine Agar with κ- and λ-Carrageenan

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1296

Composition per 1070.0mL:

Solution A…… 1.0L

Solution B…… 60.0mL

Solution C…… 10.0mL

pH 7.2 ± 0.2 at 25°C

Solution A:

Composition per liter:

NaCl ……25.0g

Agar ……15.0g

MgSO4·7H2O……5.0g

Casamino acids ……2.5g

NaNO3 ……2.0g

κ-Carrageenan……1.25g

λ-Carrageenan……1.25g

CaCl2·2H2O ……0.2g

KCl……0.1g

Preparation of Solution A: Add components to distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Gently heat and bring to boiling.

Autoclave for 15 min at 15 psi pressure –121°C.

Solution B:

Composition per 100.0mL:

Na2HPO4·2H2O……3.56g

Preparation of Solution B: Add component to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C.

Solution C:

Composition per 100.0mL:

FeSO4·7H2O……0.3g

Preparation of Solution C: Add component to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure –121°C.

Preparation of Medium: Aseptically add 60.0mL of sterile solution B and 10.0mL of sterile solution C to 1.0L of sterile solution A. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes.

Use: For the cultivation and maintenance of Pseudomonas carrageenovora.

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