海洋微生物的培养基:Marine Agar with Biphenyl
互联网
Composition per liter:
NaCl ……19.45g
Agar ……15.0g
MgCl2……8.8g
Peptone ……5.0g
Na2SO3……3.24g
CaCl2……1.8g
Yeast extract……1.0g
KCl……0.55g
NaHCO3……0.16g
Ferric citrate……0.1g
KBr ……0.08g
SrCl2……0.03g
H3BO3 ……0.02g
Na2HPO4……8.0mg
Na2SiO3……4.0mg
NaF ……2.4mg
NH4NO3 ……1.6mg
Biphenyl……1.0mg
pH 7.6 ± 0.2 at 25°C
Preparation of Medium: Add components, except biphenyl, to distilled/deionized water and bring
volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure –121°C. Pour into sterile Petri dishes or leave in tubes. After agar solidifies, aseptically add a few crystals of biphenyl to each plate.
Use: For the cultivation and maintenance of biphenyl-utilizing marine bacteria, such as Cycloclasticus pugetii.