DNA的回收与连接

互联网2013-08-27

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相关专题

实验目的

1．掌握DNA连接的的方式和影响连接反应的因素。

2．学会DNA回收和连接技术。

实验原理

将载体 pUC118 用BamHI + EcoRI酶切，

与目的基因1.0kb Bam HI + Eco RI片段用T4DNA连接酶连接起来。
DNA的回收与连接
图5-1 目的DNA与质粒连接示意图

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