丁香实验_LOGO
登录
提问
我要登录
|免费注册
点赞
收藏
wx-share
分享

Chromatin Immunoprecipitation for Studying Chromosomal Localization of Meiotic Proteins in Maize

互联网

534
Chromatin immunoprecipitation (ChIP) is a method that allows identification of chromosomal sites occupied by specific proteins. In this technique, chromatin is extracted from cells, sheared, and, using a specific antibody, enriched in fragments that contain a protein of interest. Genomic location of the protein can then be identified by hybridization of the resulting DNA to tiling microarrays or by sequencing. Thanks to advances in high-throughput sequencing methods, studying protein localization using ChIP has become possible even in species with relatively large genomes. Here, we describe a ChIP protocol that we developed to examine localization of meiotic proteins in maize.
提问
扫一扫
丁香实验小程序二维码
实验小助手
丁香实验公众号二维码
扫码领资料
反馈
TOP
打开小程序