CRESYL FAST VIOLET TECHNIQUE FOR NISSL

 

SECTIONS

8µm paraffin wax sections

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SOLUTIONS

 

STAINING SOLUTION

 

• Cresyl Fast Violet-1g
• Distilled water-100cm3
• Acetic acid-0.25cm3

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METHOD

 

1. Take sections to water
2. Stain in cresyl fast violet solution for 20-30 minutes
3. Rinse in distilled water
4. Leave in 96% alcohol until most of the stain has been removed
5. Clear in xylene. Mount sections in DPX

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RESULTS

 

• Nissl substance-Purple/dark blue
• Neurones and cell nuclei - Purple/blue