CRESYL FAST VIOLET TECHNIQUE FOR NISSL

CRESYL FAST VIOLET TECHNIQUE FOR NISSL

 

SECTIONS

8µm paraffin wax sections

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SOLUTIONS

 

STAINING SOLUTION

 

• Cresyl Fast Violet-1g
• Distilled water-100cm3
• Acetic acid-0.25cm3

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METHOD

 

1. Take sections to water
2. Stain in cresyl fast violet solution for 20-30 minutes
3. Rinse in distilled water
4. Leave in 96% alcohol until most of the stain has been removed
5. Clear in xylene. Mount sections in DPX

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RESULTS

 

• Nissl substance-Purple/dark blue
• Neurones and cell nuclei - Purple/blue

NOTE: No responsibility is assumed by The University of Nottingham or the Queens Medical Centre NHS Trust for any injury and/or damage to persons or property as a matter of products liability, negligence or otherwise, or from any use or operation of any methods, products, instructions or ideas contained in the material herein. It is the users responsibilty to ensure that all procedures are carried out according to appropriate Health and Safety requirements.