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for the technique of embedding specimens in plastic

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for the technique of embedding specimens in plastic

Day 1

Use of rubber gloves, lab coat, and safety glasses is mandatory.


Step 2 : On working mat, place the following: metal disposal dish; label to be used for specimen; specimen in 100% glycerin; paper towels; tweezers to handle specimen; paper tent (for protecting solution from dust contamination); nail to be used as pointed probe, bottle of Bio-Plastic.

Step 2a : Remove specimen from 100% glycerin and place on paper towels. Mop up as much glycerin as you are able to from the specimen.

Step 2b : Pour approximately 100ml of Bio-Plastic from the bottle to the metal disposal dish. Gently place the specimen and the label into this uncatalyzed plastic solution. Allow to remain in solution overnight. This prepares the specimen and prevents contamination by glycerin. Place the paper tent over the specimen and container to prevent contamination.

Step 3 : On working mat, place the following: mold, beaker containing water, measuring cup or ruler/tape measure for measuring the cubic centimetre(s) of the mold, pen and paper.

Step 3a : Select the mold you will use for the specimen. Calculate the volume in cubic centimetres (cc) or measure the necessary millilitres (ml) required to fill the mold. [Recall: 1cc = 1ml.]

Step 4 : On working mat, place the following: mold, mold releasing agent, paint brush, metal disposal dish; paper tent; straw or other object (if you wish to have a hole running through the plastic block); stir stick (nail); dropper (for catalyst); bottle of catalyst; bottle of Bio-Plastic; knowledge of the proper amount of Bio-Plastic and catalyst to use. See table below:

Table: Amount of chemicals required for preparing the liquid plastic.

 

 

 Type of Container

 Volume of Container

 Chemicals required (bioplastic + catalyst)

 Amount of chemical required for base layer

 Amount of chemical required for covering layer

 Pyrex glass dish  20cc or 20 ml

 Bio Plastic

Catalyst

 10 ml

8 drops

 10 ml

8 drops

 Pyrex glass dish, custard type  60cc or 60 ml

 Bio Plastic

Catalyst

 30 ml

24 drops

 30 ml

24 drops

 Metal cake tin  60cc or 60 ml

 Bio Plastic

Catalyst

 30 ml

24 drops

 30 ml

24 drops


 

Day 2

Day 3

 


stained & cleared fish

 

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