for the technique of embedding specimens in plastic

互联网2013-09-06

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for the technique of embedding specimens in plastic

Day 1

Use of rubber gloves, lab coat, and safety glasses is mandatory.

Step 2 : On working mat, place the following: metal disposal dish; label to be used for specimen; specimen in 100% glycerin; paper towels; tweezers to handle specimen; paper tent (for protecting solution from dust contamination); nail to be used as pointed probe, bottle of Bio-Plastic.

Step 2a : Remove specimen from 100% glycerin and place on paper towels. Mop up as much glycerin as you are able to from the specimen.

Step 2b : Pour approximately 100ml of Bio-Plastic from the bottle to the metal disposal dish. Gently place the specimen and the label into this uncatalyzed plastic solution. Allow to remain in solution overnight. This prepares the specimen and prevents contamination by glycerin. Place the paper tent over the specimen and container to prevent contamination.

Step 3 : On working mat, place the following: mold, beaker containing water, measuring cup or ruler/tape measure for measuring the cubic centimetre(s) of the mold, pen and paper.

Step 3a : Select the mold you will use for the specimen. Calculate the volume in cubic centimetres (cc) or measure the necessary millilitres (ml) required to fill the mold. [Recall: 1cc = 1ml.]

Step 4 : On working mat, place the following: mold, mold releasing agent, paint brush, metal disposal dish; paper tent; straw or other object (if you wish to have a hole running through the plastic block); stir stick (nail); dropper (for catalyst); bottle of catalyst; bottle of Bio-Plastic; knowledge of the proper amount of Bio-Plastic and catalyst to use. See table below:

Table: Amount of chemicals required for preparing the liquid plastic.


Type of Container	Volume of Container	Chemicals required (bioplastic + catalyst)	Amount of chemical required for base layer	Amount of chemical required for covering layer
Pyrex glass dish	20cc or 20 ml	Bio Plastic Catalyst	10 ml 8 drops	10 ml 8 drops
Pyrex glass dish, custard type	60cc or 60 ml	Bio Plastic Catalyst	30 ml 24 drops	30 ml 24 drops
Metal cake tin	60cc or 60 ml	Bio Plastic Catalyst	30 ml 24 drops	30 ml 24 drops

Day 2

Day 3

stained & cleared fish