【求助】我在genebank提交核苷酸序列遇到困难,请求帮助
丁香园论坛
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我在genebank提交核苷酸序列遇到困难,请求园内老师帮助,自己花费很大精力,可是还是存在问题。
[1] Do you know the correct annotation for your sequence(s)? If so,
please resubmit your sequence(s) with relevant features such as:
- coding regions (CDS features) including nucleotide spans
and reading frame. Using this information, our software
will add the amino acid translations for you.
- structural RNAs such as rRNAs, tRNAs, misc_RNAs, with
nucleotide spans
- features which may describe your sequence, such as
repeat_regions, UTRs, promoters with nucleotide spans
【2】下面是我提交的相关信息:
preliminary GenBank flatfiles:
LOCUS NBChina01 7414 bp RNA linear VRL 03-DEC-2010
DEFINITION Human enterovirus 71 strain NBChina01 from China,complete cds.
ACCESSION
VERSION
KEYWORDS .
SOURCE Human enterovirus 71
ORGANISM Human enterovirus 71
Viruses; ssRNA positive-strand viruses, no DNA stage;
Picornavirales; Picornaviridae; Enterovirus; Human enterovirus A.
REFERENCE 1 (bases 1 to 7414)
AUTHORS Hu,A.-r., Gu,W.-z. and Jiang,S.-w.
TITLE Autopsy report and complete genome sequence analysis of one case
with critical hang, foot and mouth disease(HFMD) infected by
enterovirus 71(EV71)
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 7414)
AUTHORS Hu,A.-r., Gu,W.-z. and Jiang,S.-w.
TITLE Direct Submission
JOURNAL Submitted (03-DEC-2010) Science and Education Division, Ningbo
Infectious Diseases Hospital, No175,Yongfeng Road(North), Ningbo
City, Zhejiang Province 315016, China
COMMENT Bankit Comment: bankit1414927.
Bankit Comment: BankIt ID:1414927.
Bankit Comment: LocalID:NBChina01.
FEATURES Location/Qualifiers
source 1..7414
/organism="Human enterovirus 71"
/proviral
/mol_type="genomic RNA"
/db_xref="taxon:39054"
/PCR_primers="fwd_name: F1, fwd_seq: ttaaaacagctgtgggttg,
fwd_name: F2, fwd_seq: atagtcggctatggtgagtg, fwd_name: F3,
fwd_seq: tgttcactgggtcctttatggc, fwd_name: F4, fwd_seq:
taccattcatgtcacctgcga, fwd_name: F5, fwd_seq:
gttttcagagcacagtttagcgg, fwd_name: F6, fwd_seq:
gtcttccagtttctttattgggctt, fwd_name: F7, fwd_seq:
ttcattctactcacgtccct, fwd_name: F8, fwd_seq:
gatcttgagtgtttcgtgcgtc, fwd_name: 3'-terminus, fwd_seq:
gacctattgtgtgcttggtgggatg, rev_name: R1, rev_seq:
acttccagtaccatcccttg, rev_name: R2, rev_seq:
agtgagtgttgctgatccatggt, rev_name: R3, rev_seq:
accagcataatttgggttggct, rev_name: R4, rev_seq:
ctagatacgacacattcccaaa, rev_name: R5, rev_seq:
gtcggcatagtgtctactggtg, rev_name: R6, rev_seq:
tgtcaaatggtatccaccgtag, rev_name: R7, rev_seq:
atcgcttagcagtcctccca, rev_name: R8, rev_seq:
cggtactgagaatcttgaggct, rev_name: 5'-terminus, rev_seq:
ggtatgtgctccgcagttagg"
/note="/organism='Human enterovirus
71'##/mol_type='genomic
RNA'##/strain='NBChina01'##/isolation_source='clinical
specimen'##/country='China:
Ningbo'##/collection_date='August-2010'"
BASE COUNT 2018 a 1791 c 1767 g 1838 t
ORIGIN
1 ttaaaacagc ctgtgggttg cacccactca cagggcccac tgggcgctag cactctggta
61 tcttggtacc tttgtgcgcc tgttttatac ccccccccag tgaaacttag aagcagcaaa
121 cctcgatcaa tagcaggcat aacgctccag ttatgtcttg atcaagcact tctgtttccc
181 cggactgagt atcaatagac tgctcgcgcg gttgaaggag aaaacgttcg ttatccggct
241 aactacttcg gaaaacctag taacaccatg gaagttgcgg agagctccgt tcagcactcc
301 cccagtgtag atcaggtcga tgagtcaccg cattccccac gggcgaccgt ggcggtggct
361 gcgttggcgg cctgcccatg gggtaaccca tggggcgctc taatacagac atggtgtgaa
421 gagtctactg agctagttag tagtcctccg gcccctgaat gcggctaatc ccaactgcgg
481 agcacacgcc cacaagccag cgggtagtgt gtcgtaacgg gtaactctgc agcggaaccg
541 actactttgg gtgtccgtgt ttccttttat ctttacattg gctgcttatg gtgacaatta
601 aagaattgtt accatatagc tattggattg gccatccggt gtgcaacaga gcaattattt
661 acctatttat tggttttgta ccatcaactt tgaattctgt gaccaccctt aattatatcc
721 tgacccttaa cacagctaaa catgggttcg caagcgtcta cacagcgctc cggttcccac
781 ggaaattcaa actcagccac tgagggttct accataaact acaccactat caattactac
841 aaagactcct atgctgccac agcaggcaaa cagagtctca agcaggatcc agacaagttt
901 gcaaatcctg ttaaatacat cttcactgaa atggcagcgc cactgaaatc cccatccgct
961 gaggcatgtg ggtacagtga tcgagtggcg caattaacta ttggcaactc cactatcacc
1021 acgcaagaag cggctaacat catagtcggt tatggcgaat ggccttccta ctgctcagat
1081 tctgacgcta cagcagtgga taaaccaacg cgcccggatg tttcagtgaa caggttttac
1141 acattggaca ctaaattgtg ggagaaatcg tccaagggat ggtactggaa gttcccggat
1201 gtgttaactg aaactggggt ttttgggcaa aatgcacaat tccactacct ctaccgatca
1261 gggttctgca tccacgtaca gtgcaatgcc agtaaattcc atcaaggagc actcctagtc
1321 gctgtcctac cagagtatgt cattgggaca gtggcaggcg gtacagggac ggaagatacc
1381 cacccccctt ataaacagac tcaacccggc gccgatggtt tcgagttgca acacccgtac
1441 gtgcttgatg ctggcatccc aatatcacag ttaacagtgt gcccacacca gtggatcaat
1501 ctaaggacca acaattgtgc tacaataata gtgccataca ttaacgcact gccttttgat
1561 tctgccttga atcattgcaa ctttggcctg ttagttgtgc ctattagccc actagactac
1621 gaccaaggag cgacgccagt aatccctata actattacat tggccccaat gtgttctgaa
1681 ttcgcaggtc ttaggcaggc agtcacgcaa gggttcccca ccgagctaaa acctggcaca
1741 aaccaatttt taaccaccga tgatggcgtt tcagcaccta ttctaccaaa cttccacccc
1801 accccgtgta tccacatacc tggtgaagtt aggaacttgc tagagttatg ccaggtggag
1861 accattctgg aggttaacaa tgtgcccacg aatgccacta gcttaatgga gagactgcgc
1921 ttcccggtct cagcacaagc agggaagggt gagctgtgtg cggtgtttag agccgatcct
1981 gggcgaagtg gaccatggca atccacctta ctgggccagt tgtgcgggta ctacacccaa
2041 tggtcaggat cattggaagt taccttcatg tttactggat ccttcatggc taccggcaag
2101 atgctcatag cctatacacc gccaggaggt cctctgccca aggaccgggc gaccgccatg
2161 ttgggcacgc acgtcatctg ggattttggg ctgcaatcgt ctgttaccct tgtaatacca
2221 tggatcagca acactcatta tagagcacat gcccgagatg gggtgtttga ctactacacc
2281 acagggttag tcagtatatg gtatcagaca aattatgtgg ttccaatcgg tgcgcccaac
2341 acagcctata taatagcatt agcggcagct caaaagaact tcactatgaa attgtgcaag
2401 gatgctagtg acatcctaca gacgggcacc atccagggag atagggtggc agatgtaata
2461 gaaagctcca taggagatag cgtgagcaga gccctcactc acgctctacc agcacccaca
2521 ggccagaaca cacaggtgag cagtcatcga ctggatacag gtaaggttcc agcactccaa
2581 gctgctgaaa ttggagcatc atcaaatgct agtgacgaga gcatgattga gacacgctgt
2641 gttcttaact cgcacagtac agctgagacc actcttgata gtttcttcag cagggcggga
2701 ttagttggag agatagatct ccctcttgag ggcacaacta acccaaatgg ttatgccaac
2761 tgggacatag atataacagg ttacgcgcaa atgcgtagaa aggtagagct attcacctac
2821 atgcgctttg atgcagagtt cacttttgtt gcgtgcacac ccaccgggga agttgttcca
2881 caaatgctcc aatatatgtt tgtgccacct ggagccccta agccagattc aagggaatcc
2941 cttgcatggc aaactgccac taacccctca gtttttgtca agctgtcaga ccctccagcg
3001 caggtttcag tgccattcat gtcacctgcg agtgcttatc aatggtttta tgacggatat
3061 cccacattcg gggaacacaa acaggagaaa gatcttgaat acggggcatg tcctaataac
3121 atgatgggca cgttctcagt gcggactgta gggacctcca agtccaagta ccctttagtg
3181 gttaggatct acatgagaat gaagcacgtc agggcgtgga tacctcgccc gatgcgtaac
3241 cagaactacc tattcaaagc caacccaaat tatgctggca actccattaa gccaactggt
3301 gctagtcgca cagcgatcac cactcttggg aaatttggac aacagtctgg ggctatttat
3361 gtgggcaact ttagagtggt caaccgacat cttgccactc acaatgattg ggcaaatctt
3421 gtttgggaag acagctctcg cgacttgctt gtgtcgtcca ccaccgccca aggttgtgac
3481 acgattgctc gctgcgattg ccagacaggg gtgtactact gtaattcgat gagaaaacac
3541 tacccagtca gtttttcaaa acccagcctg atctatgtag aggctagcga gtattaccca
3601 gccaggtacc aatcacatct catgctcgca cagggtcact cagaacctgg tgattgcggt
3661 ggtatcctta gatgccaaca tggcgtcgtc ggcatagtgt ctactggtgg taatgggctc
3721 gttggctttg cagacgtcag agacctcttg tggttagatg aagaagctat ggaacagggc
3781 gtgtccgact acatcaaggg tctcggagat gcttttggaa caggcttcac tgacgcagtc
3841 tcaagggagg ttgaagctct caaaaactat cttatagggt ctgaaggagc agttgagaaa
3901 atcttgaaaa atcttattaa actaatctct gcactggtga ttgtgatcag aagtgattac
3961 gacatggtta ccctcactgc aaccttagcg ctgataggtt gtcatggcag tccttgggct
4021 tggatcaaag ccaaaacagc ctccatctta ggtatcccta tcgctcaaaa gcagagcgct
4081 tcctggctca agaagttcaa tgacatggcc aacgccgcta aggggttaga gtgggtttcc
4141 aacaagatca gcaaatttat tgattggctt aaggagaaaa tagtaccagc ggccagggag
4201 aaggttgaat tcctaaataa cttgaaacag ctgccactgc tagagaatca gatctcaaac
4261 ttggaacagt ctgctgcctc acaagaggat cttgaagtca tgtttgggaa tgtgtcgtac
4321 ctagctcact tctgtcgcaa gttccaaccg ctatacgcca cggaagctaa aagagtctat
4381 gccctggaga agagaatgaa taactatatg cagttcaaga gcaaacaccg aattgaacct
4441 gtatgtctca ttattagggg ctcaccaggc actgggaagt ctctagccac tggtattatt
4501 gctcgagcaa ttgctgataa gtaccactcc agcgtgtact cgcttccacc agacccggat
4561 cattttgacg gttacaagca acaggtggtt acagtgatgg atgatttgtg tcaaaacccc
4621 gatggtaagg atatgtcttt attctgtcaa atggtatcca ccgtagactt cattccacca
4681 atggcttctc tcgaggagaa gggagtttcc ttcacctcta agtttgtcat cgcatccact
4741 aatgccagta atatcatagt accaacagtg tctgattctg acgctattcg ccgcaggttc
4801 tacatggact gtgacattga agtgacagac tcgtacaaaa cagatctagg tagactggat
4861 gcagggcgag ccgctaaact gtgctctgaa aacaacactg caaatttcaa acgttgcagc
4921 ccattagtgt gtgggaaagc tatccaactt agagatagaa agtctaaagt cagatacagt
4981 gtggatacgg tggtttcaga actcattagg gaatacagca ataggtccgc tattggcaac
5041 acaatcgagg ctcttttcca aggtccaccc aagttcaggc caattaggat tagccttgaa
5101 gaaaaaccag ccccagacgc tattagcgat ctccttgcta gcgtagatag tgaagaagtg
5161 cgccagtact gcagggatca aggctggatt attcctgaag ctcccaccaa tgtagagcgg
5221 caccttaata gagcggtgct tgtcatgcaa tccatcgcca cagtagtggc ggttgtctcg
5281 ttggtgtatg tcatctacaa gctctttgca gggttccagg gtgcgtattc tggtgctcct
5341 aagcaagtgc ttaagaaacc tgctcttcgc acagcgacag tacagggccc gagccttgac
5401 tttgctctct ccctactgag aaggaacatc aggcaagtcc aaacagacca ggggcatttc
5461 accatgttgg gtgttaggga tcgcttagca gtcctcccac gccactcaca acctggtaaa
5521 actatttggg ttgagcacaa actcgtgaac gtccttgatg cagttgaact ggtggatgag
5581 caaggagtca acctggaatt aaccctcatt actcttgaca ccaacgaaaa gtttagggat
5641 atcaccaaat tcatcccaga aaatattagc actgctagtg atgccaccct agtgatcaac
5701 acggagcaca tgccatcaat gtttgtcccg gtgggtgacg ttgtgcagta tggctttttg
5761 aatctcagtg gtaagcctac ccatcgcacc atgatgtaca actttcctac taaagcagga
5821 cagtgtggag gagtggtgac atctgtcggg aaggttgtcg gcattcacat tggtggcaat
5881 ggcagacaag gtttttgcgc aggcctcaaa aggagttact ttgccagtga acaaggagag
5941 atccagtggg ttaagcccaa taaagaaact ggaagactca acatcaatgg acctacccgc
6001 accaagttag aacctagtgt attccatgac atcttcgagg gaaataaaga accagctgtc
6061 ttgcacagta aagacccccg acttgaggta gactttgaac aggccctgtt ctctaagtat
6121 gtgggaaaca cactacatga gcctgacgag tacatcaaag aggcagctct tcattatgca
6181 aaccaattaa agcaactaga aattaatacc tctcaaatga gcatggagga ggcctgctac
6241 ggtactgaga atcttgaggc tattgatctt cacactagtg caggttaccc ctacagtgcc
6301 ctggggataa agaaaagaga catcttagac cctaccacca gggacgtgag tagaatgaag
6361 ttctacatgg acaagtatgg tcttgatctt ccttactcca cttatgtcaa ggacgagctg
6421 cgctcgattg ataaaatcaa gaaagggaag tcccgtctga tcgaggccag tagtctaaat
6481 gattcagtgt acctcagaat ggctttcgga catttgtatg aggctttcca cgcaaatcct
6541 gggacgataa ctggatcagc cgtggggtgt aaccctgaca cattctggag caagctgcca
6601 attttgctcc ctggttcact ctttgccttt gactactcag gttatgatgc tagccttagc
6661 cctgtctggt tcagagcatt agaattggtt cttagggaga tagggtatag tgaagaggca
6721 gtctcactca ttgagggaat caaccacaca catcatgtgt atcgtaataa gacctattgt
6781 gtgcttggtg ggatgccctc aggctgttcg ggaacgtcca ttttcaactc aatgatcaat
6841 aacattatta tcagagcact gctcgtaaaa acatttaagg gcattgattt ggatgaactc
6901 aacatggttg cttatggaga cgatgtgctc gctagctatc cctacccaat tgattgcttg
6961 gaactagcaa agactggtaa ggagtatggt ctgaccatga ctcctgctga taaatctcct
7021 tgctttaatg aggtcaattg gggtaatgcg accttcctca aaaggggctt cttgcccgat
7081 gaacagtttc catttttgat tcaccctact atgccaatga gtgagatcca tgagtccatt
7141 cgatggacca gggacgcacg aaacactcaa gatcatgtgc ggtccttgtg cctcctagca
7201 tggcataatg gtaagcaaga atatgagaag tttgtgagca caattaggtc tgtcccagta
7261 ggaagagcgt tggctatccc aaattatgaa aatcttagac gcaattggct cgaattattt
7321 tagaggttat acacacctca accccaccag aaatctggtc gtgaatgtga ctggtggggg
7381 taaatttgtt ataaccagaa tagcaaaaaa aaaa
//
【3】如果老师们可以帮助,请直接回复或者PM与我您的联系方式,不甚感谢!
[1] Do you know the correct annotation for your sequence(s)? If so,
please resubmit your sequence(s) with relevant features such as:
- coding regions (CDS features) including nucleotide spans
and reading frame. Using this information, our software
will add the amino acid translations for you.
- structural RNAs such as rRNAs, tRNAs, misc_RNAs, with
nucleotide spans
- features which may describe your sequence, such as
repeat_regions, UTRs, promoters with nucleotide spans
【2】下面是我提交的相关信息:
preliminary GenBank flatfiles:
LOCUS NBChina01 7414 bp RNA linear VRL 03-DEC-2010
DEFINITION Human enterovirus 71 strain NBChina01 from China,complete cds.
ACCESSION
VERSION
KEYWORDS .
SOURCE Human enterovirus 71
ORGANISM Human enterovirus 71
Viruses; ssRNA positive-strand viruses, no DNA stage;
Picornavirales; Picornaviridae; Enterovirus; Human enterovirus A.
REFERENCE 1 (bases 1 to 7414)
AUTHORS Hu,A.-r., Gu,W.-z. and Jiang,S.-w.
TITLE Autopsy report and complete genome sequence analysis of one case
with critical hang, foot and mouth disease(HFMD) infected by
enterovirus 71(EV71)
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 7414)
AUTHORS Hu,A.-r., Gu,W.-z. and Jiang,S.-w.
TITLE Direct Submission
JOURNAL Submitted (03-DEC-2010) Science and Education Division, Ningbo
Infectious Diseases Hospital, No175,Yongfeng Road(North), Ningbo
City, Zhejiang Province 315016, China
COMMENT Bankit Comment: bankit1414927.
Bankit Comment: BankIt ID:1414927.
Bankit Comment: LocalID:NBChina01.
FEATURES Location/Qualifiers
source 1..7414
/organism="Human enterovirus 71"
/proviral
/mol_type="genomic RNA"
/db_xref="taxon:39054"
/PCR_primers="fwd_name: F1, fwd_seq: ttaaaacagctgtgggttg,
fwd_name: F2, fwd_seq: atagtcggctatggtgagtg, fwd_name: F3,
fwd_seq: tgttcactgggtcctttatggc, fwd_name: F4, fwd_seq:
taccattcatgtcacctgcga, fwd_name: F5, fwd_seq:
gttttcagagcacagtttagcgg, fwd_name: F6, fwd_seq:
gtcttccagtttctttattgggctt, fwd_name: F7, fwd_seq:
ttcattctactcacgtccct, fwd_name: F8, fwd_seq:
gatcttgagtgtttcgtgcgtc, fwd_name: 3'-terminus, fwd_seq:
gacctattgtgtgcttggtgggatg, rev_name: R1, rev_seq:
acttccagtaccatcccttg, rev_name: R2, rev_seq:
agtgagtgttgctgatccatggt, rev_name: R3, rev_seq:
accagcataatttgggttggct, rev_name: R4, rev_seq:
ctagatacgacacattcccaaa, rev_name: R5, rev_seq:
gtcggcatagtgtctactggtg, rev_name: R6, rev_seq:
tgtcaaatggtatccaccgtag, rev_name: R7, rev_seq:
atcgcttagcagtcctccca, rev_name: R8, rev_seq:
cggtactgagaatcttgaggct, rev_name: 5'-terminus, rev_seq:
ggtatgtgctccgcagttagg"
/note="/organism='Human enterovirus
71'##/mol_type='genomic
RNA'##/strain='NBChina01'##/isolation_source='clinical
specimen'##/country='China:
Ningbo'##/collection_date='August-2010'"
BASE COUNT 2018 a 1791 c 1767 g 1838 t
ORIGIN
1 ttaaaacagc ctgtgggttg cacccactca cagggcccac tgggcgctag cactctggta
61 tcttggtacc tttgtgcgcc tgttttatac ccccccccag tgaaacttag aagcagcaaa
121 cctcgatcaa tagcaggcat aacgctccag ttatgtcttg atcaagcact tctgtttccc
181 cggactgagt atcaatagac tgctcgcgcg gttgaaggag aaaacgttcg ttatccggct
241 aactacttcg gaaaacctag taacaccatg gaagttgcgg agagctccgt tcagcactcc
301 cccagtgtag atcaggtcga tgagtcaccg cattccccac gggcgaccgt ggcggtggct
361 gcgttggcgg cctgcccatg gggtaaccca tggggcgctc taatacagac atggtgtgaa
421 gagtctactg agctagttag tagtcctccg gcccctgaat gcggctaatc ccaactgcgg
481 agcacacgcc cacaagccag cgggtagtgt gtcgtaacgg gtaactctgc agcggaaccg
541 actactttgg gtgtccgtgt ttccttttat ctttacattg gctgcttatg gtgacaatta
601 aagaattgtt accatatagc tattggattg gccatccggt gtgcaacaga gcaattattt
661 acctatttat tggttttgta ccatcaactt tgaattctgt gaccaccctt aattatatcc
721 tgacccttaa cacagctaaa catgggttcg caagcgtcta cacagcgctc cggttcccac
781 ggaaattcaa actcagccac tgagggttct accataaact acaccactat caattactac
841 aaagactcct atgctgccac agcaggcaaa cagagtctca agcaggatcc agacaagttt
901 gcaaatcctg ttaaatacat cttcactgaa atggcagcgc cactgaaatc cccatccgct
961 gaggcatgtg ggtacagtga tcgagtggcg caattaacta ttggcaactc cactatcacc
1021 acgcaagaag cggctaacat catagtcggt tatggcgaat ggccttccta ctgctcagat
1081 tctgacgcta cagcagtgga taaaccaacg cgcccggatg tttcagtgaa caggttttac
1141 acattggaca ctaaattgtg ggagaaatcg tccaagggat ggtactggaa gttcccggat
1201 gtgttaactg aaactggggt ttttgggcaa aatgcacaat tccactacct ctaccgatca
1261 gggttctgca tccacgtaca gtgcaatgcc agtaaattcc atcaaggagc actcctagtc
1321 gctgtcctac cagagtatgt cattgggaca gtggcaggcg gtacagggac ggaagatacc
1381 cacccccctt ataaacagac tcaacccggc gccgatggtt tcgagttgca acacccgtac
1441 gtgcttgatg ctggcatccc aatatcacag ttaacagtgt gcccacacca gtggatcaat
1501 ctaaggacca acaattgtgc tacaataata gtgccataca ttaacgcact gccttttgat
1561 tctgccttga atcattgcaa ctttggcctg ttagttgtgc ctattagccc actagactac
1621 gaccaaggag cgacgccagt aatccctata actattacat tggccccaat gtgttctgaa
1681 ttcgcaggtc ttaggcaggc agtcacgcaa gggttcccca ccgagctaaa acctggcaca
1741 aaccaatttt taaccaccga tgatggcgtt tcagcaccta ttctaccaaa cttccacccc
1801 accccgtgta tccacatacc tggtgaagtt aggaacttgc tagagttatg ccaggtggag
1861 accattctgg aggttaacaa tgtgcccacg aatgccacta gcttaatgga gagactgcgc
1921 ttcccggtct cagcacaagc agggaagggt gagctgtgtg cggtgtttag agccgatcct
1981 gggcgaagtg gaccatggca atccacctta ctgggccagt tgtgcgggta ctacacccaa
2041 tggtcaggat cattggaagt taccttcatg tttactggat ccttcatggc taccggcaag
2101 atgctcatag cctatacacc gccaggaggt cctctgccca aggaccgggc gaccgccatg
2161 ttgggcacgc acgtcatctg ggattttggg ctgcaatcgt ctgttaccct tgtaatacca
2221 tggatcagca acactcatta tagagcacat gcccgagatg gggtgtttga ctactacacc
2281 acagggttag tcagtatatg gtatcagaca aattatgtgg ttccaatcgg tgcgcccaac
2341 acagcctata taatagcatt agcggcagct caaaagaact tcactatgaa attgtgcaag
2401 gatgctagtg acatcctaca gacgggcacc atccagggag atagggtggc agatgtaata
2461 gaaagctcca taggagatag cgtgagcaga gccctcactc acgctctacc agcacccaca
2521 ggccagaaca cacaggtgag cagtcatcga ctggatacag gtaaggttcc agcactccaa
2581 gctgctgaaa ttggagcatc atcaaatgct agtgacgaga gcatgattga gacacgctgt
2641 gttcttaact cgcacagtac agctgagacc actcttgata gtttcttcag cagggcggga
2701 ttagttggag agatagatct ccctcttgag ggcacaacta acccaaatgg ttatgccaac
2761 tgggacatag atataacagg ttacgcgcaa atgcgtagaa aggtagagct attcacctac
2821 atgcgctttg atgcagagtt cacttttgtt gcgtgcacac ccaccgggga agttgttcca
2881 caaatgctcc aatatatgtt tgtgccacct ggagccccta agccagattc aagggaatcc
2941 cttgcatggc aaactgccac taacccctca gtttttgtca agctgtcaga ccctccagcg
3001 caggtttcag tgccattcat gtcacctgcg agtgcttatc aatggtttta tgacggatat
3061 cccacattcg gggaacacaa acaggagaaa gatcttgaat acggggcatg tcctaataac
3121 atgatgggca cgttctcagt gcggactgta gggacctcca agtccaagta ccctttagtg
3181 gttaggatct acatgagaat gaagcacgtc agggcgtgga tacctcgccc gatgcgtaac
3241 cagaactacc tattcaaagc caacccaaat tatgctggca actccattaa gccaactggt
3301 gctagtcgca cagcgatcac cactcttggg aaatttggac aacagtctgg ggctatttat
3361 gtgggcaact ttagagtggt caaccgacat cttgccactc acaatgattg ggcaaatctt
3421 gtttgggaag acagctctcg cgacttgctt gtgtcgtcca ccaccgccca aggttgtgac
3481 acgattgctc gctgcgattg ccagacaggg gtgtactact gtaattcgat gagaaaacac
3541 tacccagtca gtttttcaaa acccagcctg atctatgtag aggctagcga gtattaccca
3601 gccaggtacc aatcacatct catgctcgca cagggtcact cagaacctgg tgattgcggt
3661 ggtatcctta gatgccaaca tggcgtcgtc ggcatagtgt ctactggtgg taatgggctc
3721 gttggctttg cagacgtcag agacctcttg tggttagatg aagaagctat ggaacagggc
3781 gtgtccgact acatcaaggg tctcggagat gcttttggaa caggcttcac tgacgcagtc
3841 tcaagggagg ttgaagctct caaaaactat cttatagggt ctgaaggagc agttgagaaa
3901 atcttgaaaa atcttattaa actaatctct gcactggtga ttgtgatcag aagtgattac
3961 gacatggtta ccctcactgc aaccttagcg ctgataggtt gtcatggcag tccttgggct
4021 tggatcaaag ccaaaacagc ctccatctta ggtatcccta tcgctcaaaa gcagagcgct
4081 tcctggctca agaagttcaa tgacatggcc aacgccgcta aggggttaga gtgggtttcc
4141 aacaagatca gcaaatttat tgattggctt aaggagaaaa tagtaccagc ggccagggag
4201 aaggttgaat tcctaaataa cttgaaacag ctgccactgc tagagaatca gatctcaaac
4261 ttggaacagt ctgctgcctc acaagaggat cttgaagtca tgtttgggaa tgtgtcgtac
4321 ctagctcact tctgtcgcaa gttccaaccg ctatacgcca cggaagctaa aagagtctat
4381 gccctggaga agagaatgaa taactatatg cagttcaaga gcaaacaccg aattgaacct
4441 gtatgtctca ttattagggg ctcaccaggc actgggaagt ctctagccac tggtattatt
4501 gctcgagcaa ttgctgataa gtaccactcc agcgtgtact cgcttccacc agacccggat
4561 cattttgacg gttacaagca acaggtggtt acagtgatgg atgatttgtg tcaaaacccc
4621 gatggtaagg atatgtcttt attctgtcaa atggtatcca ccgtagactt cattccacca
4681 atggcttctc tcgaggagaa gggagtttcc ttcacctcta agtttgtcat cgcatccact
4741 aatgccagta atatcatagt accaacagtg tctgattctg acgctattcg ccgcaggttc
4801 tacatggact gtgacattga agtgacagac tcgtacaaaa cagatctagg tagactggat
4861 gcagggcgag ccgctaaact gtgctctgaa aacaacactg caaatttcaa acgttgcagc
4921 ccattagtgt gtgggaaagc tatccaactt agagatagaa agtctaaagt cagatacagt
4981 gtggatacgg tggtttcaga actcattagg gaatacagca ataggtccgc tattggcaac
5041 acaatcgagg ctcttttcca aggtccaccc aagttcaggc caattaggat tagccttgaa
5101 gaaaaaccag ccccagacgc tattagcgat ctccttgcta gcgtagatag tgaagaagtg
5161 cgccagtact gcagggatca aggctggatt attcctgaag ctcccaccaa tgtagagcgg
5221 caccttaata gagcggtgct tgtcatgcaa tccatcgcca cagtagtggc ggttgtctcg
5281 ttggtgtatg tcatctacaa gctctttgca gggttccagg gtgcgtattc tggtgctcct
5341 aagcaagtgc ttaagaaacc tgctcttcgc acagcgacag tacagggccc gagccttgac
5401 tttgctctct ccctactgag aaggaacatc aggcaagtcc aaacagacca ggggcatttc
5461 accatgttgg gtgttaggga tcgcttagca gtcctcccac gccactcaca acctggtaaa
5521 actatttggg ttgagcacaa actcgtgaac gtccttgatg cagttgaact ggtggatgag
5581 caaggagtca acctggaatt aaccctcatt actcttgaca ccaacgaaaa gtttagggat
5641 atcaccaaat tcatcccaga aaatattagc actgctagtg atgccaccct agtgatcaac
5701 acggagcaca tgccatcaat gtttgtcccg gtgggtgacg ttgtgcagta tggctttttg
5761 aatctcagtg gtaagcctac ccatcgcacc atgatgtaca actttcctac taaagcagga
5821 cagtgtggag gagtggtgac atctgtcggg aaggttgtcg gcattcacat tggtggcaat
5881 ggcagacaag gtttttgcgc aggcctcaaa aggagttact ttgccagtga acaaggagag
5941 atccagtggg ttaagcccaa taaagaaact ggaagactca acatcaatgg acctacccgc
6001 accaagttag aacctagtgt attccatgac atcttcgagg gaaataaaga accagctgtc
6061 ttgcacagta aagacccccg acttgaggta gactttgaac aggccctgtt ctctaagtat
6121 gtgggaaaca cactacatga gcctgacgag tacatcaaag aggcagctct tcattatgca
6181 aaccaattaa agcaactaga aattaatacc tctcaaatga gcatggagga ggcctgctac
6241 ggtactgaga atcttgaggc tattgatctt cacactagtg caggttaccc ctacagtgcc
6301 ctggggataa agaaaagaga catcttagac cctaccacca gggacgtgag tagaatgaag
6361 ttctacatgg acaagtatgg tcttgatctt ccttactcca cttatgtcaa ggacgagctg
6421 cgctcgattg ataaaatcaa gaaagggaag tcccgtctga tcgaggccag tagtctaaat
6481 gattcagtgt acctcagaat ggctttcgga catttgtatg aggctttcca cgcaaatcct
6541 gggacgataa ctggatcagc cgtggggtgt aaccctgaca cattctggag caagctgcca
6601 attttgctcc ctggttcact ctttgccttt gactactcag gttatgatgc tagccttagc
6661 cctgtctggt tcagagcatt agaattggtt cttagggaga tagggtatag tgaagaggca
6721 gtctcactca ttgagggaat caaccacaca catcatgtgt atcgtaataa gacctattgt
6781 gtgcttggtg ggatgccctc aggctgttcg ggaacgtcca ttttcaactc aatgatcaat
6841 aacattatta tcagagcact gctcgtaaaa acatttaagg gcattgattt ggatgaactc
6901 aacatggttg cttatggaga cgatgtgctc gctagctatc cctacccaat tgattgcttg
6961 gaactagcaa agactggtaa ggagtatggt ctgaccatga ctcctgctga taaatctcct
7021 tgctttaatg aggtcaattg gggtaatgcg accttcctca aaaggggctt cttgcccgat
7081 gaacagtttc catttttgat tcaccctact atgccaatga gtgagatcca tgagtccatt
7141 cgatggacca gggacgcacg aaacactcaa gatcatgtgc ggtccttgtg cctcctagca
7201 tggcataatg gtaagcaaga atatgagaag tttgtgagca caattaggtc tgtcccagta
7261 ggaagagcgt tggctatccc aaattatgaa aatcttagac gcaattggct cgaattattt
7321 tagaggttat acacacctca accccaccag aaatctggtc gtgaatgtga ctggtggggg
7381 taaatttgtt ataaccagaa tagcaaaaaa aaaa
//
【3】如果老师们可以帮助,请直接回复或者PM与我您的联系方式,不甚感谢!
如果老师们可以帮助,请直接回复或者PM与我您的联系方式,不甚感谢!
hehe,是要你提供序列注释。你如何得到该序列的?ORF起止位置?有没有重复区?有没有非翻译区?有没有启动子?这样大家用你提供的序列才能方便。
其实你如果按BANKIT活SEQIN的步骤一步步来,应该不会漏掉的。你现在可通过RESUBMISSION修改。
如果实在有困难可直接回信请求帮助,要求他们帮你。
其实你如果按BANKIT活SEQIN的步骤一步步来,应该不会漏掉的。你现在可通过RESUBMISSION修改。
如果实在有困难可直接回信请求帮助,要求他们帮你。
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