荧光标记基团的激发和发射波长是广大科研工作者最关心的内容.
下面就我们大家常用的多肽标记的各种荧光基团数据参数提供给大家.
荧光染料激发波长，nm发射波长，nm
FITC494518
5-FAM494522
TAMRA560582
Rhodamine B555580
Cy3550570
Cy5649670
从发射波长相近的角度考虑,上述荧光基团在用AlexFluor替换时,可参照下表
Alexa Fluor dyeAlternatives common dyes
Alexa Fluor 350Coumarin (MCA, AMC)
Alexa Fluor 488Cy2 or Fluorescein (FITC, FAM)
Alexa Fluor 555Cy3 or Tetramethylrhodamine (TRMRA)
Alexa Fluor 568Rhodamine Red
Alexa Fluor 594Texas Red
Alexa Fluor 647Cy5
Alexa Fluor 680Cy5.5
Alexa Fluor 750Cy7
| 荧光分子 | 颜色 | 激发波长 | 发射波长 |
| Alexa Fluor 350 | Blue | 346 nm | 442 nm |
| Alexa Fluor 405 | Blue | 402 nm | 421 nm |
| Pacific Blue | Blue | 410 nm | 455 nm |
| DAPI | Blue | 340 nm | 488 nm |
| AMCA | Blue | 350 nm | 450 nm |
| Cascade Blue | Blue | 400 nm | 420 nm |
| EviTag(TM) quantum dots - Lake Placid Blue (490 nm) | Blue | <400 nm | 490 nm |
| Fluoroblue | Blue | 350 nm | 450 nm |
| Image-Blue | Blue | 380 nm | 465 nm |
| Marina Blue | Blue | 365 nm | 460 nm |
| Pacific Blue | Blue | 410 nm | 455 nm |
| Cy2 | Green | 491 nm | 509 nm |
| Alexa Fluor 430 | Green | 434 nm | 539 nm |
| Alexa Fluor 488 | Green | 495 nm | 519 nm |
| Alexa Fluor 488 FluoroNanogold | Green | 495 nm | 519 nm |
| Alexa Fluor 500 | Green | 503 nm | 525 nm |
| Alexa Fluor 514 | Green | 518 nm | 540 nm |
| FITC | Green | 490 nm | 520 nm |
| Fluorescein | Green | 494 nm | 518 nm |
| HNP | Green | 290 nm | 509 nm |
| 5-FAM | Green | 494 nm | 522 nm |
| 6-FAM | Green | 492 nm | 517 nm |
| BODIPY FL | Green | 505 nm | 513 nm |
| CMNB-caged Fluorescein | Green | 494 nm | 518 nm |
| Cyanine 2 | Green | 492 nm | 510 nm |
| DTAF | Green | 495 nm | 520 nm |
| DTAF/FITC | Green | 495 nm | 520 nm |
| dUTP-Biotin and Avidin FITC | Green | 495 nm | 520 nm |
| dUTP-FITC | Green | 495 nm | 520 nm |
| EviTag(TM) quantum dots - Adirondack Green (520 nm) | Green | <400 nm | 520 nm |
| EviTag(TM) quantum dots - Catskill Green (540 nm) | Green | <400 nm | 540 nm |
| FITC (Fluorescein) | Green | 495 nm | 520 nm |
| Fluorescein-5-EX, succinimidyl ester (FSE) | Green | 495 nm | 520 nm |
| Hi FITC | Green | 495 nm | 520 nm |
| JOE | Green | 520 nm | 548 nm |
| MFP488 | Green | 501 nm | 523 nm |
| Oregon Green 488 | Green | 496 nm | 524 nm |
| Oregon Green 514 | Green | 511 nm | 530 nm |
| Qdot 525 | Green | 488 nm | 515 nm |
| TET | Green | 521 nm | 536 nm |
| CAL Fluor(R) Gold 540 | Green | 522 nm | 544 nm |
| NL493 | Green | 493 nm | 514 nm |
| GFP | Green | 395/475 nm | 509 nm |
| Cy3 | Yellow | 548 nm | 562 nm |
| Cy3B | Yellow | 558 nm | 572 nm |
| Alexa Fluor 532 | Yellow | 531 nm | 554 nm |
| Alexa Fluor 546 | Yellow | 556 nm | 573 nm |
| Alexa Fluor 555 | Yellow | 555 nm | 565 nm |
| R-Phycoerythrin | Yellow | 480, 546, 565 nm | 578 nm |
| B-Phycoerythrin | Yellow | 546, 565 nm | 575 nm |
| HNP/Fast Red TR | Yellow | 290 nm | 562 nm |
| 5-TAMRA | Yellow | 546 nm | 574 nm |
| Atto 550 | Yellow | 554 nm | 576 nm |
| CAL Fluor(R) Orange 560 | Yellow | 538 nm | 559 nm |
| Cyanine 3 | Yellow | 550 nm | 570 nm |
| DyLight 547 | Yellow | 547 nm | 574 nm |
| EviTag(TM) quantum dots - Birch Yellow (580 nm) | Yellow | <400 nm | 580 nm |
| EviTag(TM) quantum dots - Hops Yellow (560 nm) | Yellow | <400 nm | 560 nm |
| HEX | Yellow | 535 nm | 556 nm |
| NED | Yellow | 546 nm | 575 nm |
| Qdot 565 | Yellow | <500 nm | 565 nm |
| Quasar(R) 570 | Yellow | 548 nm | 566 nm |
| R-Phycoerythrin (R-PE) | Yellow | 488 nm | 575 nm |
| TRITC (Rhodamine) | Yellow | 550 nm | 570 nm |
| VIC | Yellow | 538 nm | 554 nm |
| NL557 | Yellow | 557 nm | 574 nm |
| Phycoerythrin | Yellow | 480-566 nm | 575 nm |
| Cy3.5 | Orange | 581 nm | 596 nm |
| Alexa Fluor 568 | Orange | 578 nm | 603 nm |
| ROX | Orange | 575 nm | 602 nm |
| DyeMer 488/605 | Orange | 502 nm | 609 nm |
| DyeMer 488/615 | Orange | 502 nm | 615 nm |
| CAL Fluor(R) Red 590 | Orange | 569 nm | 591 nm |
| CAL Fluor(R) Red 610 | Orange | 569 nm | 610 nm |
| Cyanine 3.5 | Orange | 581 nm | 596 nm |
| DyeMer 488/605 | Orange | 488 nm | 605 nm |
| DyeMer 488/615 | Orange | 488 nm | 615 nm |
| EviTag(TM) quantum dots - Fort Orange (600 nm) | Orange | <400 nm | 600 nm |
| Lissamine Rhodamine | Orange | 570 nm | 590 nm |
| MFP555 | Orange | 560 nm | 585 nm |
| Qdot 585 | Orange | 532 nm | 585 nm |
| Qdot 605 | Orange | <600 nm | 605 nm |
| ROX | Orange | 575 nm | 602 nm |
| R-Phycoerythrin-Texas Red (PE-Texas Red, ECD) | Orange | 480-565 nm | 615 nm |
| Rhodamine Red-X | Orange | 570 nm | 590 nm |
| TMR red | Red | 540 (520-560) nm | 580 (570-620) nm |
| Cy5 | Red | 646 nm | 664 nm |
| Cy5.5 | Red | 673 nm | 692 nm |
| CypHer5E | Red | 644 nm | 663 nm |
| Alexa Fluor 594 | Red | 590 nm | 617 nm |
| Alexa Fluor 594 FluoroNanogold | Red | 590 nm | 617 nm |
| Alexa Fluor 610 | Red | 612 nm | 628 nm |
| Alexa Fluor 610–R-PE | Red | 496, 546, 565 nm | 630 nm |
| Alexa Fluor 633 | Red | 632 nm | 647 nm |
| Alexa Fluor 635 | Red | 633 nm | 647 nm |
| Alexa Fluor 647 | Red | 650 nm | 668 nm |
| Alexa Fluor 647–R-phycoerythrin | Red | 496, 546, 565 nm | 668 nm |
| Alexa Fluor 660 | Red | 663 nm | 690 nm |
| Texas Red | Red | 596 nm | 620 nm |
| DyeMer 488/630 | Red | 502 nm | 630 nm |
| Allophycocyanin | Red | 650 nm | 660 nm |
| PI | Red | 535 nm | 617 nm |
| Allophycocyanin (APC) | Red | 650 nm | 660 nm |
| Allophycocyanin-Cyanine 5 | Red | 633 nm | 670 nm |
| Allophycocyanin-Cyanine 5.5 | Red | 633 nm | 690 nm |
| Atto 647 | Red | 645 nm | 669 nm |
| CAL Fluor(R) Red 635 | Red | 618 nm | 637 nm |
| Cyanine 5 | Red | 650 nm | 670 nm |
| DyLight 647 | Red | 647 nm | 673 nm |
| DyeMer 488/630 | Red | 488 nm | 630 nm |
| Dyomics 647 | Red | 660 nm | 673 nm |
| Lightcycler Red- 640 | Red | 625 nm | 640 nm |
| PBXL-1 | Red | 545 nm | 666 nm |
| PBXL-3 | Red | 614 nm | 662 nm |
| PerCP (Peridinin Chlorophyll) | Red | 490 nm | 675 nm |
| PerCP-Cy5.5 | Red | 488 nm | 695 nm |
| Qdot 655 | Red | <620 nm | 655 nm |
| Quantum Red | Red | 488 nm | 660 nm |
| Quasar(R) 670 | Red | 647 nm | 667 nm |
| R-Phycocyanin (R-PC) | Red | 614 nm | 647 nm |
| R-Phycoerythrin-Cyanine 5 (Cy-Chrome, PC5, Tri-Color, Spectral Red) | |
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