常用荧光染料的激发及发射波长
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Fluorescent Dye (荧光染料) |
Excitation |
Emission |
Cy2 TM |
489 |
506 |
GFP(Red Shifted) |
488 |
507 |
YO-PRO TM -1 |
491 |
509 |
YOYO TM -1 |
491 |
509 |
Calcein |
494 |
517 |
FITC |
494 |
518 |
FluorX TM |
494 |
519 |
Alexa TM 488 |
490 |
520 |
Rhodamine 110 |
496 |
520 |
ABI,5-FAM |
494 |
522 |
Oregon Green TM 500 |
503 |
522 |
Oregon Green TM 488 |
496 |
524 |
RlboGreen TM |
500 |
525 |
Rhodamine Green TM |
502 |
527 |
Rhodamine123 |
507 |
529 |
Magnesium Green TM |
506 |
531 |
Calcium Green TM |
506 |
533 |
TO-PRO TM -1 |
514 |
533 |
TOTO?-1 |
514 |
533 |
ABI,JOE |
520 |
548 |
BODIPY?530/550 |
530 |
550 |
Dil |
549 |
565 |
BODIPY?TMR |
542 |
568 |
BODIPY?558/568 |
558 |
568 |
BODIPY?564/570 |
564 |
570 |
Cy3 TM |
550 |
570 |
Alexa TM 546 |
555 |
570 |
TRITC |
547 |
572 |
Magnesium Orange TM |
550 |
575 |
Phycoerythrin,R & B |
565 |
575 |
Rhodamine Phalloidin |
550 |
575 |
Calcium Orange TM |
549 |
576 |
Pyronin Y |
555 |
580 |
Rhodamine B |
555 |
580 |
ABI,TAMRA |
560 |
582 |
Rhodamine Red TM |
570 |
590 |
Cy3.5 TM |
581 |
596 |
ABI,ROX |
588 |
608 |
Calcium Crimson TM |
590 |
615 |
Alexa TM 594 |
590 |
615 |
Texas Red? |
595 |
615 |
Nile Red |
549 |
628 |
YO-PRO TM -3 |
612 |
631 |
YOYO TM -3 |
612 |
631 |
R-Phycocyanin |
618 |
642 |
C-Phycocyanin |
620 |
648 |
TO-PRO TM -3 |
642 |
660 |
TOTO?-3 |
642 |
660 |
DiD DilC’(5) |
644 |
665 |
Cy5 TM |
649 |
670 |
Thiadicarbocyanine |
651 |
671 |
Cy5.5 |
675 |
694 |