染色质 免疫沉淀 反应
互联网
|
Chromatin Immunoprecipitation Protocols. ChIP assay. Protocols and Methods for chromatin IP.
CHROMATIN IMMUNOPRECIPITATION (CHIP) PROTOCOL FOR YEAST PDF - http://research.stowers-institute.org/jeg/2004/cohesin/protocols/ChIP.pdf
Protocol derived from a paper by Miriam Braunstein and is based on work from the Allis lab.
Chromatin Immunoprecipitation (ChIPs) Cloning Protocol - http://genomecenter.ucdavis.edu/farnham/farnham/protocols/ChIPscloning.htmlChromatin Immunoprecipitation (ChIPs) Cloning Protocol Farnham Lab
Chromatin Immunoprecipitation (ChIPs) Protocol - http://genomecenter.ucdavis.edu/farnham/farnham/protocols/chips.htmlIP Wash buffer recipes and protocol for ChIP Assay. Protocol works with NIH 3T3, Friend, HeLa, Raji and CHO cells.. Farnham Lab
Chromatin Immunoprecipitation (ChIPs) Protocol for Tissues - http://genomecenter.ucdavis.edu/farnham/farnham/protocols/tissues.htmlChromatin Immunoprecipitation (ChIPs) Protocol for Tissues. Farnham Lab.
Chromatin Immunoprecipitation Protocol - /attach/2008/2008-02-01/FF/FF194C6609B9F73761BD4D317C7D1BCC.pdfChIP Staph A cell preparation, preblocking and ChIP IP preclear, recovery, wash steps based on Farnham Lab Protocols.
Chromatin Immunoprecipitation Protocol PDF - /attach/2008/2008-02-01/68/688F4AE554C045ACA17A9EFB848F3703.pdfChromatin Immunoprecipitation Protocol for Microarray Analysis �Protein A/G Bead Method. Cross-Linking, Sonication/Chromatin Shearing Efficiency. Reaction UHN Microarray Centre
Chromatin IP (CHIP assay) - http://www.fhcrc.org/science/labs/breeden/Methods/chromatinIP.htmlChIP assay protocol with 2 steps: in vivo formaldehyde cross-linking of whole cells and protein-protein and protein-DNA interactions, followed by immunoprecipitation of protein-DNA complexes with specific antibodies from sonicated extracts. Breeden Lab
Chromatin-IP (ChrIP) Protocol - http://www.ncbi.nlm.nih.gov/books/bv.fcgi?rid=inserm.section.340Mapping Protein/DNA Interactions by Cross-Linking Examining the Distribution of Telomeric and DNA Repair Proteins by ChrIP and Real-Time PCR
Expert protocols and tips for N-ChIP and X-ChIP. - http://www.abcam.com/index.html?pageconfig=resource&rid=10418&CFID=2422773&CFTOKEN=36329956Expert protocols and tips for N-ChIP and X-ChIP. AbCAM
Immunoprecipitation of Chromatin from Fixed Yeast Protocol PDF - http://derisilab.ucsf.edu/pdfs/BeadBeatIP_Protocol.pdfChromatin-IP (or ChIP) has been a useful method for the localization of proteins to specific regions of DNA. ChIP applied to microarrays (ChIP on Chip) Brown Lab
Mirmira Lab ChIP Protocol - http://faculty.virginia.edu/mirmira/resources_files/Protocols/MirmiraLabChIPprotocol.htmMirmira Lab ChIP Protocol. 50-step protocol forformaldehyde crosslinking to ChIP and also provides recipes for all buffer reagents.
Native Chromatin Immunoprecipitation Protocol - http://www.abcam.com/index.html?pageconfig=resource&rid=30&pid=5Native Chromatin Immunoprecipitation Protocol ABCAM
Yeast Chromatin Immunoprecipitation (ChIP) - http://www.fhcrc.org/science/labs/hahn/methods/mol_bio_meth/hahnlab_ChIP_method.htmlChromatin IP Method, ChIP buffers, Notes on ChIP Method, Quantative PCR using 32P dCTP. Hahn Lab.
ChIP Assay Protocol PDF - /attach/2008/2008-02-01/2B/2B6C4EA25F257424710A50E83CB2F380.pdfFormaldehyde cross-linking and chromatin immunoprecipitation assays of tissue culture cells, Based on Boyd and Farnham. Michelle Kallesen, Rosen Lab.
ChIP on ChIP Protocol PDF - /attach/2008/2008-02-01/D0/D04E8898B377B49D51F27E282E4D7A79.pdfMethod to identify genomic targets of DNA-binding factors. Chromatin immunoprecipitation (ChIP) assay on high-throughput microarray based methods for discovering genomic regions occupied by human DNA-binding proteins. Oberley and Farnham.
ChIP ping for Dummies - http://mcardle.oncology.wisc.edu/sugden/Protocols/html%20files/ChIPping%20for%20Dummies.htmVery nice and detailed ChIP assay protocol procedure. Blocking Staph A cells, Crosslinking cells, Chromatin preparation from cell pellets, sonication, blocking. Sugden Laboratory
ChIP Protocol - http://www.genetics.ucla.edu/labs/fan/Protocols_ChIP.htmChIP Protocol. Fan Lab UCLA.
ChIP Tips and FAQs - http://www.abcam.com/index.html?rid=310ChIP Tips and FAQs. AbCam.
ChIP TIPS PDF - /attach/2008/2008-02-01/19/19CE6B9E7B1E1CCD5C6B9AF1D7DD3CAB.pdfPrepare Chromatin, Immunoprecipitate, Wash, Elute, IP Procedure. UpState.
CHROMATIN IMMUNOPRECIPITATION (CHIP) PROTOCOL FOR YEAST - http://www.ciwemb.edu/labs/koshland/Protocols/YEAST/chipmod.htmlSPHEROPLAST CELLS, WASH SPHEROPLASTS, SONICATE SPHEROPLASTS, CHROMATIN SOLUTION, SET UP IMMUNOPRECIPITATIONS, REVERSE FORMALDEHYDE CROSSLINKS. Paul - Koshland lab.