【求助】MitoTracker® Red CM-H2XRos(7513)检测的是什么
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请问MitoTracker® Red CM-H2XRos(7513)检测的是什么啊？网站上说氧化后才有荧光，那么检测的是ROS吗？但是网站上又说染料的积累与膜电位相关，各位有没有人知道它到底检测的是什么？谢谢！
简言之，该探针目前应用就是针对检测线粒体的，是一种改良的探针，优点：易于操作、固定、联合标记且更加稳定
你这个问题的认识过程如下：
1、什么是MitoTracker® probes ？
Molecular Probes’ MitoTracker® probes are a series of novel mitochondrion selective dyes that are well retained during cell fixation. Conventional fluorescent stains for mitochondria — such as tetramethylrosamine and rhodamine 123 — are readily sequestered by the actively respiring organelles; however, they are subsequently washed out of cells once the mitochondria’s membrane potential is lost. This characteristic severely limits their use in experiments in which cells must be treated with aldehyde fixatives or other agents that affect the energetic state of the mitochondria.
The cell-permeant MitoTracker probes contain a thiol-reactive chloromethyl moiety. Once the MitoTracker probe accumulates in the mitochondria, it can react with accessible thiol groups on peptides and proteins to form an aldehyde-fixable
conjugate .
2.MitoTracker® probes优点：
The orange and red MitoTracker dyes are retained after permeabilization, and cells stained with these probes can be fixed, permeabilized and then double-labeled with an anti-body directed against a mitochondrial protein or other cellular marker. Our MitoTracker reagents should also eliminate some of the hazards of working with pathogenic cells because, once the mitochondria are stained, the pathogenic cells can be treated with fixatives before the sample is analyzed.
3.MitoTracker® probes的当前产品主要成员？
M-7510:orange-fluorescent tetramethylrosamine
M-7511:nonfluorescent reduced form of M-7510 , dihydrotetramethylrosamine,a probe that is oxidized to a fluorescent product within the mitochondria.
M-7512:chloromethyl derivatives of X-rosamine ，which label mitochondria with a
longer-wavelength fluorescence emission that is well resolved from fluorescein’s emission.
M-7513：见4
These aboved four MitoTracker probes accumulate in active mitochondria, are well retained after cell fixation and, unlike the MitoTracker Green FM, are well retained after permeabilization.
M-7514：MitoTracker Green FM
3.具体到MitoTracker® Red CM-H2XRos(7513)又如何？
MitoTracker Red CM-H2XRos is a reduced, nonfluorescent version of MitoTracker Red (M-7512) that fluoresces upon oxidation. This dye also stains mitochondria in live cells and its accumulation is dependent upon membrane potential. The dye is well-retained after aldehyde fixation.
你这个问题的认识过程如下：
1、什么是MitoTracker® probes ？
Molecular Probes’ MitoTracker® probes are a series of novel mitochondrion selective dyes that are well retained during cell fixation. Conventional fluorescent stains for mitochondria — such as tetramethylrosamine and rhodamine 123 — are readily sequestered by the actively respiring organelles; however, they are subsequently washed out of cells once the mitochondria’s membrane potential is lost. This characteristic severely limits their use in experiments in which cells must be treated with aldehyde fixatives or other agents that affect the energetic state of the mitochondria.
The cell-permeant MitoTracker probes contain a thiol-reactive chloromethyl moiety. Once the MitoTracker probe accumulates in the mitochondria, it can react with accessible thiol groups on peptides and proteins to form an aldehyde-fixable
conjugate .
2.MitoTracker® probes优点：
The orange and red MitoTracker dyes are retained after permeabilization, and cells stained with these probes can be fixed, permeabilized and then double-labeled with an anti-body directed against a mitochondrial protein or other cellular marker. Our MitoTracker reagents should also eliminate some of the hazards of working with pathogenic cells because, once the mitochondria are stained, the pathogenic cells can be treated with fixatives before the sample is analyzed.
3.MitoTracker® probes的当前产品主要成员？
M-7510:orange-fluorescent tetramethylrosamine
M-7511:nonfluorescent reduced form of M-7510 , dihydrotetramethylrosamine,a probe that is oxidized to a fluorescent product within the mitochondria.
M-7512:chloromethyl derivatives of X-rosamine ，which label mitochondria with a
longer-wavelength fluorescence emission that is well resolved from fluorescein’s emission.
M-7513：见4
These aboved four MitoTracker probes accumulate in active mitochondria, are well retained after cell fixation and, unlike the MitoTracker Green FM, are well retained after permeabilization.
M-7514：MitoTracker Green FM
3.具体到MitoTracker® Red CM-H2XRos(7513)又如何？
MitoTracker Red CM-H2XRos is a reduced, nonfluorescent version of MitoTracker Red (M-7512) that fluoresces upon oxidation. This dye also stains mitochondria in live cells and its accumulation is dependent upon membrane potential. The dye is well-retained after aldehyde fixation.
谢谢，版主，我也正在找
我也需要这个可以高价购买，邮箱hongkun999@163.com
有帮助，要是有试剂说明书就更好了
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