组织学――组织制备

 

・         Histological techniques (William H. Heidcamp)
Very detailed guide to histological techniques, like  fixation, dehydration, embedment and subsequent sectioning.
  

・         3-Aminopropyltriethoxysilane Treatment of Slides (Nottingham Pathology Lab)
  

・         Paraffin-Embedded Tissue for LCM (Arcturus)
Paraffin embedding is a process in which fixed (formalin or other fixative) is infiltrated and then blocked out in paraffin to stabilize it for long-term storage and easy sectioning...
   

・         Wax embedding/sectioning (Klymkowsky Lab)
  

• Chrome Gelatinised Slides and Coverslips (Nottingham Pathology Lab)
     
• Decalcification (Nottingham Pathology Lab)
  Many surgical specimens contain calcified areas which need to be decalcified before processing and sectioning. This is the protocol used for this purpose
    
• De-Paraffinization of Tissue Sections (Arcturus)
  Remove paraffin from section for tissue preparation such as nucleic acid extraction.
     
• DNA Extraction From Paraffin Section (Arcturus)
  This extraction method has been used for measuring loss of heterozygosity (LOH), dideoxy fingerprinting (DDF), clonality analysis (chromosome X inactivation) and direct sequencing of PCundefined products for single base mutational analysis. Extractions are typically performed on 500-1000 captured cells.
    
• RNA Extraction from Paraffin Section (NIH Protocol) (Arcturus)
  A Protocol for extracting RNA from paraffin embedded tissue.
    
• Preparation of Frozen Tissue for LCM (Arcturus)
    
• White Blood Cell Cytospin (Arcturus)