【求助】抗体制备服务
丁香园论坛
2373
请问各位大侠，有没有订做过磷酸化的丝氨酸或磷酸化的苏氨酸抗体，哪家公司做的比较好，小弟想订做目的蛋白特异位点的丝氨酸、苏氨酸的磷酸化的抗体，帮忙给指点下，多谢啦
这种抗体定做很难。毕竟要的是抗一个特异的氨基酸的磷酸化位点。
就我所知cisbio有anti-phosphothronine 和anti-phosphotyrosine的抗体，PAb和MAb都有，但是没有anti-phosphoserine的抗体。
摘抄：
Anti-Phosphothreonine (MAb) - CryptateThe anti-phosphothreonine antibody is a monoclonal antibody (clone 42H4) produced at Cell Signaling Technology (reference 9386). It recognizes phosphorylated threonine residues in a manner largely independent of the surrounding amino-acid sequence. The antibody is phospho-specific but does not cross-react with phospho-serine-containing sequences. The anti-phosphothreonine monoclonal antibody is labeled with Eu3+ Cryptate by Cisbio international. Specific activity of the conjugate ranges from 4 to 7 Cryptates per antibody. This conjugate is supplied in 50 mM hepes buff er pH 7.0, 0.1% bovine serum albumin (BSA) protease free.
The concentration of this conjugate has been calibrated in order to obtain a 620 nm signal within the 30,000-50,000 count range. This calibration was run in a 384-well low volume plate format using a final volume of 20 μL, a buffer containing 0.1% BSA and 0.4M KF, and a RUBYstar reader (BMG LABTECH). A recommended amount of antibody per well is specified in the product information sheet attached to the product. On this basis, each vial enables the assessment of 500 tests. Actual amount per well will be dependent on optimized assay conditions and miniaturization.
这个抗体是接了Eu的，该公司用作HTRF试剂。
从介绍中可以得知，该抗体为CST产品，故CST可能也有类似抗体。
就我所知cisbio有anti-phosphothronine 和anti-phosphotyrosine的抗体，PAb和MAb都有，但是没有anti-phosphoserine的抗体。
摘抄：
Anti-Phosphothreonine (MAb) - CryptateThe anti-phosphothreonine antibody is a monoclonal antibody (clone 42H4) produced at Cell Signaling Technology (reference 9386). It recognizes phosphorylated threonine residues in a manner largely independent of the surrounding amino-acid sequence. The antibody is phospho-specific but does not cross-react with phospho-serine-containing sequences. The anti-phosphothreonine monoclonal antibody is labeled with Eu3+ Cryptate by Cisbio international. Specific activity of the conjugate ranges from 4 to 7 Cryptates per antibody. This conjugate is supplied in 50 mM hepes buff er pH 7.0, 0.1% bovine serum albumin (BSA) protease free.
The concentration of this conjugate has been calibrated in order to obtain a 620 nm signal within the 30,000-50,000 count range. This calibration was run in a 384-well low volume plate format using a final volume of 20 μL, a buffer containing 0.1% BSA and 0.4M KF, and a RUBYstar reader (BMG LABTECH). A recommended amount of antibody per well is specified in the product information sheet attached to the product. On this basis, each vial enables the assessment of 500 tests. Actual amount per well will be dependent on optimized assay conditions and miniaturization.
这个抗体是接了Eu的，该公司用作HTRF试剂。
从介绍中可以得知，该抗体为CST产品，故CST可能也有类似抗体。
多谢euthor_hsu的指点，现已在CST找到磷酸化苏氨酸抗体。
euthor_hsu wrote:
这种抗体定做很难。毕竟要的是抗一个特异的氨基酸的磷酸化位点。
就我所知cisbio有anti-phosphothronine 和anti-phosphotyrosine的抗体，PAb和MAb都有，但是没有anti-phosphoserine的抗体。
摘抄：
Anti-Phosphothreonine (MAb) - CryptateThe anti-phosphothreonine antibody is a monoclonal antibody (clone 42H4) produced at Cell Signaling Technology (reference 9386). It recognizes phosphorylated threonine residues in a manner largely independent of the surrounding amino-acid sequence. The antibody is phospho-specific but does not cross-react with phospho-serine-containing sequences. The anti-phosphothreonine monoclonal antibody is labeled with Eu3+ Cryptate by Cisbio international. Specific activity of the conjugate ranges from 4 to 7 Cryptates per antibody. This conjugate is supplied in 50 mM hepes buff er pH 7.0, 0.1% bovine serum albumin (BSA) protease free.
The concentration of this conjugate has been calibrated in order to obtain a 620 nm signal within the 30,000-50,000 count range. This calibration was run in a 384-well low volume plate format using a final volume of 20 μL, a buffer containing 0.1% BSA and 0.4M KF, and a RUBYstar reader (BMG LABTECH). A recommended amount of antibody per well is specified in the product information sheet attached to the product. On this basis, each vial enables the assessment of 500 tests. Actual amount per well will be dependent on optimized assay conditions and miniaturization.
这个抗体是接了Eu的，该公司用作HTRF试剂。
从介绍中可以得知，该抗体为CST产品，故CST可能也有类似抗体。
euthor_hsu战友说的很对。
不过cisbio有抗磷酸化的丝氨酸，苏氨酸和酪氨酸的抗体。具体你可以看以下这个网址：http://www.htrf.com/products/kinase_oncology/kinease/
如果你需要更详细的资料可以PM我:D
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