bamboopiggy
TTACCAATGCTTAATCAG 我直接在vectorNTI中计算的是37.7
汤姆卜丽波
粗略算法: Tm=4×(G+C)+2×(A+T) 或者用primer5(or oligo6)计算
灵枢天问
(G—C)%=(Tm-69.3)×2.44
在一定条件下(pH7.0,0.165MNaCl中)Tm值与(GC)%含量呈正比关系。
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