Construction of Multiply Disabled Herpes Simplex Viral Vectors for Gene Delivery to the Nervous System
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This chapter aims to describe the main procedures involved in constructing a disabled recombinant herpes simplex virus type 1 (HSV-1) vector. However, before describing the methodology in detail, it is important to briefly consider the structure and life cycle of HSV1 and the modifications that must be made to the virus in order to generate an efficient gene delivery vector.
