Giemsa-trypsin Banding

试剂

Earles Balanced Salt Solution (BSS) earles

Fetal Bovine Serum
Giemsa stain, original azure blend Giemsa
Harleco 620G/75 harleco 620g/75
Gurrs buffer tablets, pH 6.8 gurrs
Biomedical Specialties, Santa Monica,
Trypsin solution
GIBCO, Cat. gibco
Water, sterile

制备

Trypsin working solution

Trypsin solution, 2.5% 1.5 ml
Earles BSS 50 ml earles
Fetal bovine serum solution
Fetal bovine serum 1 ml
Earles BSS 50ml earles
Gurrs buffer solution, pH 6.8 gurrs
Dissolve 1 tablet in 1 L sterile water
Giema Stain working solution
Giemsa stain 1 ml Giemsa
Gurrs buffer solution, pH 6.8 50 ml