【共享】 RNAi资源放送
丁香园论坛
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内容包括：
1＝＝＝＝humanaebook：RNA Interference, Editing, and Modification
Abstract:
A comprehensive collection of cutting-edge methods for elucidating the function of new genes and altering gene expression. These readily reproducible techniques can be used either in transient and stable gene splicing applied to worms, flies, trypanosomes, mammals, and plants, or in studying RNA editing mechanisms in a wide range of organisms, including systems that involve the conversion of one base to another and insertion/deletion editing. Topics of interest include stable and transient RNA interference, gene silencing, RNA editing, bioinformatics, small noncoding RNAs, and RNomics. Special attention is given to methods for the identification and characterization of small RNAs involved in RNA interference or modification.
2 ＝＝＝Nature Biotechnology 5周年RNAi专题
包括：
1 Synthetic shRNAs as potent RNAi triggers
2 Synthetic dsRNA Dicer substrates enhance RNAi potency and efficacy.◎
3 Knockdown by RNAi-proceed with caution
4 Rational siRNA design for RNA interference
5 Lack of interferon response in animals to naked siRNAs.◎
6 RNAi-mediated replacement of morphine with the nonnarcotic alkaloid reticuline in opium poppy
7 Approaches for the sequence-specific knockdown of mRNA.◎
8 RNAi targeting of DNA virus in plants.◎
9 Screening for gene function in chicken embryo using RNAi and electroporation.◎
10 Recombinant Dicer efficiently converts large dsRNAs into siRNAs suitable for gene silencing
11 Inhibition of hepatitis B virus in mice by RNA interference.◎
12 Transgenic RNA interference in ES cell-derived embryos recapitulates a genetic null phenotype.
13 Expression profiling reveals off-target gene regulation by RNAi.◎
14 Unlocking the money-making potential of RNAi
15 Why RNAi makes sense.◎
16 Effective expression of small interfering RNA in human cells.◎
17 U6 promoter-driven siRNAs with four uridine 3' overhangs efficiently suppress targeted gene expression in mammalian cells.◎
18 Expression of small interfering RNAs targeted against HIV-1 rev transcripts in human cells.◎
19 Heritable gene silencing in Drosophila using double-stranded RNA.◎
分享地点：
＝＝＝1
http://www.k65.net/u.php?n=ZHFsb3Zlcj0v
密码：02170625（04.17～05.16有效）
＝＝＝2
本版公共邮箱
1＝＝＝＝humanaebook：RNA Interference, Editing, and Modification
Abstract:
A comprehensive collection of cutting-edge methods for elucidating the function of new genes and altering gene expression. These readily reproducible techniques can be used either in transient and stable gene splicing applied to worms, flies, trypanosomes, mammals, and plants, or in studying RNA editing mechanisms in a wide range of organisms, including systems that involve the conversion of one base to another and insertion/deletion editing. Topics of interest include stable and transient RNA interference, gene silencing, RNA editing, bioinformatics, small noncoding RNAs, and RNomics. Special attention is given to methods for the identification and characterization of small RNAs involved in RNA interference or modification.
2 ＝＝＝Nature Biotechnology 5周年RNAi专题
包括：
1 Synthetic shRNAs as potent RNAi triggers
2 Synthetic dsRNA Dicer substrates enhance RNAi potency and efficacy.◎
3 Knockdown by RNAi-proceed with caution
4 Rational siRNA design for RNA interference
5 Lack of interferon response in animals to naked siRNAs.◎
6 RNAi-mediated replacement of morphine with the nonnarcotic alkaloid reticuline in opium poppy
7 Approaches for the sequence-specific knockdown of mRNA.◎
8 RNAi targeting of DNA virus in plants.◎
9 Screening for gene function in chicken embryo using RNAi and electroporation.◎
10 Recombinant Dicer efficiently converts large dsRNAs into siRNAs suitable for gene silencing
11 Inhibition of hepatitis B virus in mice by RNA interference.◎
12 Transgenic RNA interference in ES cell-derived embryos recapitulates a genetic null phenotype.
13 Expression profiling reveals off-target gene regulation by RNAi.◎
14 Unlocking the money-making potential of RNAi
15 Why RNAi makes sense.◎
16 Effective expression of small interfering RNA in human cells.◎
17 U6 promoter-driven siRNAs with four uridine 3' overhangs efficiently suppress targeted gene expression in mammalian cells.◎
18 Expression of small interfering RNAs targeted against HIV-1 rev transcripts in human cells.◎
19 Heritable gene silencing in Drosophila using double-stranded RNA.◎
分享地点：
＝＝＝1
http://www.k65.net/u.php?n=ZHFsb3Zlcj0v
密码：02170625（04.17～05.16有效）
＝＝＝2
本版公共邮箱
