RNA点线杂交

RNA 点线杂交（ Dot and Slot blotting ）

・         RNA dot blot and slot blot ( Beverly Faulkner-Jones)
This methods allows the rapid analysis of numerous small samples for the sequence of interest and is less time consuming than the gel electrophoresis methods. 'Dots' or 'slots' of RNA are made onto a filter using a manifold and the filter is then hybridized with a labeled probe. Partially degraded RNA can also be used with good semi-quantitative results. However, high false positive rates need careful use of appropriate controls