HLA-DR基因中等分辨率分型序列特异性引物
互联网
1278
HLA-DR 基因中等分辨率分型序列特异性引物
5’-引物
|
序列(5’ � 3’)
|
3’-引物
|
序列(5’ � 3’)
|
PCR产物长度
|
DRB1基因扩增特异性
|
5’01
|
TTGTGGCAGCTTAAGTTTGAAT
|
3’047
|
CTGCACTGTGAAGCTCGCAC
|
255bp
|
0101-0102
|
|
|
3’048
|
CTGCACACTGTGAAGCTCTCCA
|
255bp
|
|
5’01
|
TTGTGGCAGCTTAAGTTTGAAT
|
3’10
|
CCCGCTCGTCTTCCAGGAT
|
130bp
|
0103
|
5’02
|
tcctgtggcagcctaagag
|
3’01
|
CCGCGCCTGCTCCAGGATT
|
197bp
|
1501,1502
|
5’02
|
tcctgtggcagcctaagag
|
3’03
|
AGGTGTCCACCGCGGCG
|
213bp
|
1601,1602
|
5’03
|
TACTTCCATAACCAGGAGGAGA
|
3’03
|
TGCAGTAGTTGTCCACCCG
|
151bp
|
0301,0302
|
5’06
|
GACGGAGCCGGTGCGTA
|
3’48
|
CTGCACACTGTGAAGCTCTCCA
|
217bp
|
0301
|
5’03
|
TACTTCCATAACCAGGAGGAGA
|
3’47
|
CTGCACTGTGAAGCTCGCAC
|
189bp
|
0302,1302,1305
|
|
|
|
|
|
1402,1403,1409
|
5’04
|
GTTTCTTGGAGCAGGTTAAACA
|
3’47
|
CTGCACTGTGAAGCTCGCAC
|
260bp
|
0401 -0411
|
|
|
3’048
|
CTGCACACTGTGAAGCTCTCCA
|
260bp
|
|
5’07
|
CCTGTGGCAGGGTAAGTATA
|
3’079
|
CCCGTAGTTGTGTCTCGACAC
|
232bp
|
0701,0702
|
5’08
|
ACTACTCTACGGGTGAGTGTT
|
3’05
|
CTGCAGTAGGTGTCCACCAG
|
214bp
|
0801-0806
|
5’09
|
gtttcttgaagcaggataagttt
|
3’079
|
CCCGTAGTTGTGTCTCGACAC
|
236bp
|
0901
|
5’10
|
gttcttggagtactctacgtc
|
3’047
|
CTGCACTGTGAAGCTCGCAC
|
204bp
|
1001
|
5’05
|
gttCTTGGAGTACTCTACGTC
|
3’06
|
CTGGCTGTTCCAGTACTCCT
|
176bp
|
1101 - 1105
|
5’08
|
ACTACTCTACGGGTGAGTGTT
|
3’08C
|
TCTGTGAAGCTCTCCACAG
|
248bp
|
1201,1202
|
5’03
|
TACTTCCATAACCAGGAGGAGA
|
3’10
|
CCCGCTCGTCTTCCAGGAT
|
130bp
|
1301,1302
|
5’05
|
gttCTTGGAGTACTCTACGTC
|
3’11
|
TCTGCAATAGGTGTCCACCT
|
224bp
|
1401,1404,1405
|
5’08
|
ACTACTCTACGGGTGAGTGTT
|
|
|
|
1407,1408
|
5’03
|
TACTTCCATAACCAGGAGGAGA
|
3’12
|
TCCACCGCGGCCCTCC
|
140bp
|
1402,1404,1409
|
|
|
|
|
|
1305,1306
|
5’04
|
GTTTCTTGGAGCAGGTTAAACA
|
3’19
|
ctgttccagtctccgcag
|
188bp
|
1410
|
5’52.1
|
TTTCTTGGAGCTGCGTAAGTC
|
3’13
|
CTGTTCCAGGATCGGGCGA
|
171bp
|
DRBundefined0101 - 0301
|
5’52.2
|
GTTTCTTGGAGCTGCTTAAG
|
3’14
|
GCTGTTCCAGTACTCGGCAT
|
173bp
|
|
5’53
|
GAGCGAGTGTGGACCTGA
|
3’048
|
CTGCACACTGTGAAGCTCTCCA
|
213bp
|
DRBundefined0101
|
5’51
|
GTTTCTTGCAGCCAGGATAAGTA
|
3’01
|
CCGCGCCTGCTCCAGGATT
|
198bp
|
DRBundefined0101 �0 202
|
|
|
3’16
|
CCGCGGCGGCCTGTCT
|
207bp
|
|