In Vivo Libraries of Large Insert Transgenic Mice for Genetic Mapping

Genetic mapping of phenotypic traits in mammals has historically relied on meiotic recombination together with the use of polymorphic markers. Some examples of these markers, in chronological order, are coat color alleles, protein polymorphisms, restriction fragment length polymorphisms (RFLP) (1 ,2 ), microsatellite repeats (simple sequence length polymorphisms, or SSLPs) (3 ,4 ), and most recently single nucleotide polymorphisms (SNPs) arrayed on chips (5 ). These approaches have been employed to map both single-gene mutations and genes involved in complex traits.