Sequencing of Double-Stranded PCR Products

The polymerase chain reaction (PCR) is fast becoming a standard protocol in many laboratories for cloning and analysis of small quantities of DNA (1 ). It involves the exponential synthesis of linear DNA molecules from double-stranded DNA templates using specific oligonucleotide primers for the DNA synthesis reaction. Because of its unique sensitivity and general applicability, the PCR procedure has been used in a variety of systems.