细胞株的Ⅳ型胶原酶测定

问：有人提到一种酶缓冲液提供酶反应条件，酶蛋白即催化底物明胶，不知道是怎么回事情。我要测定活性和分泌。我知道是要使用聚丙烯酰胺凝胶电泳的，请教具体的实验方法很所需要的试剂。谢谢！

答：Type IV collagenase, 72-kD, is officially designated matrix metalloproteinase-2 (MMP2). It is also known as gelatinase, 72-kD. Type IV collagenase is a metalloproteinase that specifically cleaves type IV collagen, the major structural component of basement membranes. The metastatic potential of tumor cells has been found to correlate with the activity of this enzyme.

常规的作作明胶酶谱即可，请参贴子
http://www.dxy.cn/bbs/post/view?bid=41&id=107511&sty=3&keywords=%C3%B8%C6%D7