Silver Staining Protocol
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1x 40min - overnight 50% MeOH, 12% Acetic Acid
1x 30min 50% MeOH, 12% Acetic Acid, 0.05% 37% Formaldehyde
3x 20min 50% ETOH
1x 0.2min (variable) 200 mg/l Na2 S2 O3 .5H2 O
2x 2-3min ddH2 O
1x 20min 2 g/l AgNO3 , 750ul/l 37% Formaldehyde
1x 2-3min ddH2 O
variable 60 g/l Na2 CO3 , 5 mg/l Na2 S2 O3 .5H2 O, 500ul/l 37% Formaldehyde
1x 2-3min ddH2 O
1x 10min 50% MeOH, 12% Acetic Acid
1x 30min overnight 20% ETOH, 2% Glycerol