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Affinity Immunoblotting

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2227
Chapter 9 developed the concept of antibody clonality and described several methods that have been used to define antibody clonality in polyclonal sera. Affinity immunoblotting (1 ) has proven to be a powerful and high-resolution method for estimating clonality of immune responses to a variety of antigens. This chapter describes the affinity immunoblotting method in detail.
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