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Hematopoietic Stem Cell Development During Mouse Embryogenesis

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173
The progress of the last few years in the understanding of hematopoietic cell development during embryogenesis resulted from a combination of experimental approaches used in hematology and developmental biology. This methodology has been particularly powerful for the analysis of the earliest steps of hematopoietic ontogeny because it allows for the first time the demonstration of the existence of two independent sites of hematopoietic cell generation. Here, we describe the methods used in our laboratories to characterize the phenotype and differentiation potential of the primordial hematopoietic precursors as well as their localization in the mouse embryo. This multidisciplinary approach is required to explore the mechanisms of hematopoietic cell generation.
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