Nuclear Magnetic Resonance Structural Studies of Membrane Proteins in Micelles and Bilayers
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Nuclear magnetic resonance (NMR) spectroscopy enables determination of membrane protein structures in lipid environments, such as micelles and bilayers. This chapter outlines the steps for membrane-protein structure determination using solution NMR with micelle samples, and solid-state NMR with oriented lipid-bilayer samples. The methods for protein expression and purification, sample preparation, and NMR experiments are described and illustrated with examples from γ and CHIF, two membrane proteins that function as regulatory subunits of the Na+ - and K+ -ATPase.
