Nuclear Magnetic Resonance Studies of Oligonucleotides

NMR spectroscopy is an extremely valuable and versatile method for studies of nucleosides, nucleotides, and their oligomers. It can provide information about chemical composition, three-dimensional structures, internal motions, and interactions with other macromolecules or ligands. It can be used to follow outcome and yield of a synthesis, to ascertain identity and purity of the product, to identify and quantify byproducts, and finally, to obtain a largely complete picture of the spatial arrangement of atoms in the molecule. It can be used at all stages of synthesis, development, and investigations of oligonucleotides.