配制Tris-甘氨酸SDS聚丙烯酰胺凝胶电泳分离胶所用溶液
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配制Tris-甘氨酸SDS聚丙烯酰胺凝胶电泳分离胶所用溶液
溶液成分
|
不同体积(ml)凝胶液中各成分所需体积(ml)
|
|||||||
5
|
10
|
15
|
20
|
25
|
30
|
40
|
50
|
|
6%
|
|
|
|
|
|
|
|
|
水
|
2.6
|
5.3
|
7.9
|
10.6
|
13.2
|
15.9
|
21.2
|
26.5
|
30%丙烯酰胺溶液
|
1.0
|
2.0
|
3.0
|
4.0
|
5.0
|
6.0
|
8.0
|
10.0
|
1.5mol/L Tris-HCl,pH 8.8
|
1.3
|
2.5
|
3.8
|
5.0
|
6.3
|
7.5
|
10.0
|
12.5
|
10%(w/v)SDS
|
0.03
|
0.10
|
0.15
|
0.20
|
0.25
|
0.30
|
0.40
|
0.50
|
10%过硫酸胺
|
0.05
|
0.10
|
0.15
|
0.20
|
0.25
|
0.30
|
0.40
|
0.50
|
TEMED
|
0.004
|
0.008
|
0.012
|
0.016
|
0.02
|
0.024
|
0.032
|
0.04
|
8%
|
|
|
|
|
|
|
|
|
水
|
2.3
|
4.6
|
6.9
|
9.3
|
11.5
|
13.9
|
18.5
|
23.2
|
30%丙烯酰胺溶液
|
1.3
|
2.7
|
4.0
|
5.3
|
6.7
|
8.0
|
10.7
|
13.3
|
1.5mol/L Tris-HCl,pH 8.8
|
1.3
|
2.5
|
3.8
|
5.0
|
6.3
|
7.5
|
10.0
|
12.5
|
10%(w/v)SDS
|
0.03
|
0.10
|
0.15
|
0.20
|
0.25
|
0.30
|
0.40
|
0.50
|
10%过硫酸胺
|
0.05
|
0.10
|
0.15
|
0.20
|
0.25
|
0.30
|
0.40
|
0.50
|
TEMED
|
0.004
|
0.008
|
0.012
|
0.016
|
0.02
|
0.024
|
0.032
|
0.04
|
10%
|
|
|
|
|
|
|
|
|
水
|
1.9
|
4.0
|
5.9
|
7.9
|
9.9
|
11.9
|
15.9
|
19.8
|
30%丙烯酰胺溶液
|
1.7
|
3.3
|
5.0
|
6.7
|
8.3
|
10.0
|
13.3
|
16.7
|
1.5mol/L Tris-HCl,pH 8.8
|
1.3
|
2.5
|
3.8
|
5.0
|
6.3
|
7.5
|
10.0
|
12.5
|
10%(w/v)SDS
|
0.03
|
0.10
|
0.15
|
0.20
|
0.25
|
0.30
|
0.40
|
0.50
|
10%过硫酸胺
|
0.05
|
0.10
|
0.15
|
0.20
|
0.25
|
0.30
|
0.40
|
0.50
|
TEMED
|
0.004
|
0.008
|
0.012
|
0.016
|
0.02
|
0.024
|
0.032
|
0.04
|
12%
|
|
|
|
|
|
|
|
|
水
|
1.6
|
3.3
|
4.9
|
6.6
|
8.2
|
9.9
|
13.2
|
16.5
|
30%丙烯酰胺溶液
|
2.0
|
4.0
|
6.0
|
8.0
|
10.0
|
12.0
|
16.0
|
20.0
|
1.5mol/L Tris-HCl,pH 8.8
|
1.3
|
2.5
|
3.8
|
5.0
|
6.3
|
7.5
|
10.0
|
12.5
|
10%(w/v)SDS
|
0.03
|
0.10
|
0.15
|
0.20
|
0.25
|
0.30
|
0.40
|
0.50
|
10%过硫酸胺
|
0.05
|
0.10
|
0.15
|
0.20
|
0.25
|
0.30
|
0.40
|
0.50
|
TEMED
|
0.004
|
0.008
|
0.012
|
0.016
|
0.02
|
0.024
|
0.032
|
0.04
|
15%
|
|
|
|
|
|
|
|
|
水
|
1.1
|
2.3
|
3.4
|
4.6
|
5.7
|
6.9
|
9.2
|
11.5
|
30%丙烯酰胺溶液
|
2.5
|
5.0
|
7.5
|
10.0
|
12.5
|
15.0
|
20.0
|
25.0
|
1.5mol/L Tris-HCl,pH 8.8
|
1.3
|
2.5
|
3.8
|
5.0
|
6.3
|
7.5
|
10.0
|
12.5
|
10%(w/v)SDS
|
0.03
|
0.10
|
0.15
|
0.20
|
0.25
|
0.30
|
0.40
|
0.50
|
10%过硫酸胺
|
0.05
|
0.10
|
0.15
|
0.20
|
0.25
|
0.30
|
0.40
|
0.50
|
TEMED
|
0.004
|
0.008
|
0.012
|
0.016
|
0.02
|
0.024
|
0.032
|
0.04
|