丁香实验_LOGO
登录
提问
我要登录
|免费注册
点赞
收藏
wx-share
分享

mRNA Detection in Cerebral Vessels by Nonradioactive In Situ Hybridization

互联网

512
In situ hybridization (ISH) has become a critical tool for studying gene expression in the central nervous system (CNS) (1 6 ). ISH has advantages over immunohisto-chemistry, because ISH identifies the cells that make the antigen of interest, rather than just contain it. ISH has been used to assess the cellular distribution of messenger ribonucleic acids (mRNAs) encoding many important proteins during neural embryogenesis, and in reactive and neoplastic pathological conditions in the brain (6 10 ). In recent years, the sensitivity of ISH techniques has increased, using both radioactive and nonradioactive probes to allow the detection of mRNAs that encode various molecules (3 ,5 ,6 ,10 17 ) and there is increasing use of nonradioactive ISH to identify a number of genes related to vasculogenesis and angiogenesis in the brain (18 24 ).
提问
扫一扫
丁香实验小程序二维码
实验小助手
丁香实验公众号二维码
扫码领资料
反馈
TOP
打开小程序