Organogenesis in Callus Culture

Plant cells can be totipotent, i.e., each cell may be capable of developing into an entire plant when provided with the correct environmental stimuli. Research during the last 30 yr has demonstrated that successful organogenesis in callus cultures can be achieved by the correct choice of medium components, selection of a suitable inoculum, and control of the physical environment (1 ). The manipulation of plant growth regulator concentration is probably the most widely used technique for the induction of organogenesis, and this methodology has formed the basis of the propagation of commercially important plants via tissue culture in recent years (2 ).