【求助】室温放置10余天的标本是否还能进行PCR、western
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我做的过敏性鼻炎小鼠模型,鼻粘膜取后一直放在-80度中,但至少半个月前被人误拿出而未收好,即在室温中放置了至少半个月:(
请教各位高手,这些标本是否还能进行real time PCR、western blot。本人半年多的心血,舍不得放弃。
谢谢大家的宝贵意见,同时祝您新年快乐,万事顺心!
请教各位高手,这些标本是否还能进行real time PCR、western blot。本人半年多的心血,舍不得放弃。
谢谢大家的宝贵意见,同时祝您新年快乐,万事顺心!
genomic PCR should be more or less ok ; qRT-PCR couold be problematic.
Western blot , your interested protein could be degradable , so ...
But why not just try once. To be positive if samples are precious.
Western blot , your interested protein could be degradable , so ...
But why not just try once. To be positive if samples are precious.
Thank you for your advice!
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