blast这样结果的一对引物能用吗？
丁香园论坛
2060
我想要的是大鼠GAPDH的一对引物，做内参用。blast出来那么多similar，能用吗？
引物序列
AGTTCAACGGCACAGTCAAGG
anti
CGCCAGTAGACTCCACGACAT
Sequences producing significant alignments: (bits) Value
gi|22857817|gb|AC122039.4| Mus musculus BAC clone RP24-432H... 44 0.013
gi|38089166|ref|XM_124904.2| Mus musculus similar to glycer... 44 0.013
gi|38086567|ref|XM_355889.1| Mus musculus similar to glycer... 44 0.013
gi|38084956|ref|XM_124767.3| Mus musculus similar to glycer... 44 0.013
gi|38077614|ref|XM_354907.1| Mus musculus similar to glycer... 44 0.013
gi|34869414|ref|XM_221353.2| Rattus norvegicus similar to g... 44 0.013
gi|34869269|ref|XM_213595.2| Rattus norvegicus similar to g... 44 0.013
gi|34875026|ref|XM_213530.2| Rattus norvegicus similar to g... 44 0.013
gi|34873038|ref|XM_220863.2| Rattus norvegicus similar to g... 44 0.013
gi|34867302|ref|XM_343299.1| Rattus norvegicus similar to g... 44 0.013
gi|27719050|ref|XM_235346.1| Rattus norvegicus similar to g... 44 0.013
gi|34865770|ref|XM_345831.1| Rattus norvegicus similar to g... 44 0.013
gi|34864974|ref|XM_345816.1| Rattus norvegicus similar to g... 44 0.013
gi|34864318|ref|XM_343421.1| Rattus norvegicus similar to g... 44 0.013
gi|34935492|ref|XM_345722.1| Rattus norvegicus similar to G... 44 0.013
gi|34935357|ref|XM_234405.2| Rattus norvegicus similar to g... 44 0.013
gi|34869858|ref|XM_342865.1| Rattus norvegicus similar to g... 44 0.013
gi|34881824|ref|XM_219822.2| Rattus norvegicus similar to g... 44 0.013
gi|34882876|ref|XM_214537.2| Rattus norvegicus similar to g... 44 0.013
gi|34868407|ref|XM_342841.1| Rattus norvegicus similar to g... 44 0.013
gi|34858282|ref|XM_216453.2| Rattus norvegicus hypothetical... 44 0.013
gi|34855937|ref|XM_342697.1| Rattus norvegicus similar to g... 44 0.013
gi|34860251|ref|XM_342556.1| Rattus norvegicus similar to g... 44 0.013
gi|34859499|ref|XM_230775.2| Rattus norvegicus similar to g... 44 0.013
gi|34851362|ref|XM_226328.2| Rattus norvegicus similar to g... 44 0.013
gi|34858813|ref|XM_342527.1| Rattus norvegicus similar to g... 44 0.013
gi|34878353|ref|XM_226094.2| Rattus norvegicus similar to K... 44 0.013
gi|34877369|ref|XM_225604.2| Rattus norvegicus similar to g... 44 0.013
gi|34853386|ref|XM_345334.1| Rattus norvegicus similar to G... 44 0.013
gi|34879670|ref|XM_225042.2| Rattus norvegicus similar to g... 44 0.013
gi|34877741|ref|XM_341422.1| Rattus norvegicus similar to g... 44 0.013
gi|34875552|ref|XM_224472.2| Rattus norvegicus similar to g... 44 0.013
gi|34875520|ref|XM_224464.2| Rattus norvegicus similar to g... 44 0.013
gi|34852784|ref|XM_342166.1| Rattus norvegicus similar to g... 44 0.013
gi|34879625|ref|XM_223717.2| Rattus norvegicus similar to g... 44 0.013
gi|34861964|ref|XM_219750.2| Rattus norvegicus similar to g... 44 0.013
gi|34877345|ref|XM_214046.2| Rattus norvegicus similar to g... 44 0.013
gi|34875902|ref|XM_213991.2| Rattus norvegicus similar to g... 44 0.013
gi|34879481|ref|XM_213867.2| Rattus norvegicus similar to g... 44 0.013
gi|34879275|ref|XM_232707.2| Rattus norvegicus similar to g... 44 0.013
gi|37590766|gb|BC059110.1| Rattus norvegicus glyceraldehyde... 44 0.013
gi|33942196|gb|AC102494.6| Mus musculus chromosome 7, clone... 44 0.013
gi|34368616|gb|AC125565.5| Rattus norvegicus 2 BAC CH230-9H... 44 0.013
gi|21218401|gb|AF512009.1| Sigmodon hispidus glyceraldehyde... 44 0.013
gi|27356781|gb|AY066007.1| Meriones unguiculatus glyceralde... 44 0.013
gi|56187|emb|X02231.1|RNGADPHR Rattus norvegicus mRNA for g... 44 0.013
gi|32452393|dbj|AB040445.1| Meriones unguiculatus GAPDH mRN... 44 0.013
gi|28189708|dbj|AB098979.1| Bos taurus mRNA for similar to ... 44 0.013
gi|28189618|dbj|AB098934.1| Bos taurus mRNA for similar to ... 44 0.013
gi|28189564|dbj|AB098907.1| Bos taurus mRNA for similar to ... 44 0.013
gi|2285902|emb|AJ000039.1|BTGAPDH Bos taurus mRNA for glyce... 44 0.013
gi|1841757|gb|U85042.1|BTU85042 Bos taurus glyceraldehyde-p... 44 0.013
gi|10190788|gb|AF106860.2|AF106860 Rattus norvegicus glycer... 44 0.013
gi|9798637|dbj|AB017801.1| Rattus norvegicus mRNA for glyce... 44 0.013
gi|1675348|gb|U75401.1|RNU75401 Rattus norvegicus glycerald... 44 0.013
gi|31377487|ref|NM_017008.2| Rattus norvegicus glyceraldehy... 44 0.013
gi|22137354|gb|BC024703.1| Mus musculus RIKEN cDNA 3000002C... 42 0.051
gi|34870654|ref|XM_340786.1| Rattus norvegicus similar to g... 42 0.051
gi|34865813|ref|XM_343455.1| Rattus norvegicus similar to g... 42 0.051
gi|34867135|ref|XM_218090.2| Rattus norvegicus similar to g... 42 0.051
gi|34879837|ref|XM_217067.2| Rattus norvegicus similar to g... 42 0.051
gi|34933053|ref|XM_233295.2| Rattus norvegicus similar to g... 42 0.051
gi|34852539|ref|XM_215391.2| Rattus norvegicus similar to g... 42 0.051
gi|34852531|ref|XM_342144.1| Rattus norvegicus similar to g... 42 0.051
gi|34857537|ref|XM_215798.2| Rattus norvegicus similar to g... 42 0.051
gi|34879542|ref|XM_341616.1| Rattus norvegicus similar to G... 42 0.051
gi|34858143|ref|XM_227423.2| Rattus norvegicus similar to g... 42 0.051
gi|34855494|ref|XM_215526.2| Rattus norvegicus similar to G... 42 0.051
gi|34853006|ref|XM_215441.2| Rattus norvegicus similar to g... 42 0.051
gi|34879714|ref|XM_223741.2| Rattus norvegicus similar to g... 42 0.051
gi|34880932|ref|XM_213945.2| Rattus norvegicus similar to g... 42 0.051
gi|45120379|gb|AC147251.3| Mus musculus BAC clone RP24-114L... 42 0.051
gi|45237291|gb|AC140286.3| Mus musculus BAC clone RP24-573E... 42 0.051
gi|28189720|dbj|AB098985.1| Bos taurus mRNA for similar to ... 42 0.051
gi|39752877|gb|AC123950.3| Mus musculus BAC clone RP23-162I... 42 0.051
gi|204248|gb|M17701.1|RATGAPDHA Rat glyceraldehyde-3-phosph... 42 0.051
gi|21531039|emb|AL645640.14| Mouse DNA sequence from clone ... 42 0.051
gi|38327333|gb|AY363102.1|AY363102S1 Mus musculus diadenosi... 40 0.20
gi|37574271|gb|AC146815.1| Mus musculus BAC clone RP23-239A... 40 0.20
gi|38371912|gb|AC101886.16| Mus musculus chromosome 15, clo... 40 0.20
gi|27356748|gb|AC121959.3| Mus musculus BAC clone RP24-247N... 40 0.20
gi|29373194|gb|AC125177.4| Mus musculus BAC clone RP23-315H... 40 0.20
gi|22138613|gb|AC121838.2| Mus musculus BAC clone RP24-74O1... 40 0.20
gi|38173991|gb|BC061210.1| Mus musculus cDNA clone IMAGE:30... 40 0.20
gi|38092293|ref|XM_354654.1| Mus musculus similar to glycer... 40 0.20
gi|33569140|emb|AL845336.9| Mouse DNA sequence from clone R... 40 0.20
gi|25815334|gb|AC091683.52| Mus musculus chromosome 13 clon... 40 0.20
gi|46359985|gb|AC131323.9| Mus musculus chromosome 17, clon... 40 0.20
gi|46359983|gb|AC124113.9| Mus musculus chromosome 5, clone... 40 0.20
gi|33146035|emb|AL954379.17| Mouse DNA sequence from clone ... 40 0.20
gi|45356249|gb|AC121279.7| Mus musculus chromosome 3, clone... 40 0.20
gi|31043743|emb|AL831714.14| Mouse DNA sequence from clone ... 40 0.20
gi|30140547|emb|AL935328.14| Mouse DNA sequence from clone ... 40 0.20
gi|28625316|emb|AL929179.6| Mouse DNA sequence from clone R... 40 0.20
gi|25013342|gb|AC087416.18| Mus musculus chromosome 2 clone... 40 0.20
gi|41470583|gb|AC115705.13| Mus musculus chromosome 3, clon... 40 0.20
gi|41393315|gb|AC105297.35| Mus musculus clone rp23-14k19 m... 40 0.20
gi|40352726|gb|BC064681.1| Mus musculus similar to glyceral... 40 0.20
gi|41054967|ref|NM_199472.1| Mus musculus similar to glycer... 40 0.20
gi|24474460|emb|AL672180.11| Mouse DNA sequence from clone ... 40 0.20
引物序列
AGTTCAACGGCACAGTCAAGG
anti
CGCCAGTAGACTCCACGACAT
Sequences producing significant alignments: (bits) Value
gi|22857817|gb|AC122039.4| Mus musculus BAC clone RP24-432H... 44 0.013
gi|38089166|ref|XM_124904.2| Mus musculus similar to glycer... 44 0.013
gi|38086567|ref|XM_355889.1| Mus musculus similar to glycer... 44 0.013
gi|38084956|ref|XM_124767.3| Mus musculus similar to glycer... 44 0.013
gi|38077614|ref|XM_354907.1| Mus musculus similar to glycer... 44 0.013
gi|34869414|ref|XM_221353.2| Rattus norvegicus similar to g... 44 0.013
gi|34869269|ref|XM_213595.2| Rattus norvegicus similar to g... 44 0.013
gi|34875026|ref|XM_213530.2| Rattus norvegicus similar to g... 44 0.013
gi|34873038|ref|XM_220863.2| Rattus norvegicus similar to g... 44 0.013
gi|34867302|ref|XM_343299.1| Rattus norvegicus similar to g... 44 0.013
gi|27719050|ref|XM_235346.1| Rattus norvegicus similar to g... 44 0.013
gi|34865770|ref|XM_345831.1| Rattus norvegicus similar to g... 44 0.013
gi|34864974|ref|XM_345816.1| Rattus norvegicus similar to g... 44 0.013
gi|34864318|ref|XM_343421.1| Rattus norvegicus similar to g... 44 0.013
gi|34935492|ref|XM_345722.1| Rattus norvegicus similar to G... 44 0.013
gi|34935357|ref|XM_234405.2| Rattus norvegicus similar to g... 44 0.013
gi|34869858|ref|XM_342865.1| Rattus norvegicus similar to g... 44 0.013
gi|34881824|ref|XM_219822.2| Rattus norvegicus similar to g... 44 0.013
gi|34882876|ref|XM_214537.2| Rattus norvegicus similar to g... 44 0.013
gi|34868407|ref|XM_342841.1| Rattus norvegicus similar to g... 44 0.013
gi|34858282|ref|XM_216453.2| Rattus norvegicus hypothetical... 44 0.013
gi|34855937|ref|XM_342697.1| Rattus norvegicus similar to g... 44 0.013
gi|34860251|ref|XM_342556.1| Rattus norvegicus similar to g... 44 0.013
gi|34859499|ref|XM_230775.2| Rattus norvegicus similar to g... 44 0.013
gi|34851362|ref|XM_226328.2| Rattus norvegicus similar to g... 44 0.013
gi|34858813|ref|XM_342527.1| Rattus norvegicus similar to g... 44 0.013
gi|34878353|ref|XM_226094.2| Rattus norvegicus similar to K... 44 0.013
gi|34877369|ref|XM_225604.2| Rattus norvegicus similar to g... 44 0.013
gi|34853386|ref|XM_345334.1| Rattus norvegicus similar to G... 44 0.013
gi|34879670|ref|XM_225042.2| Rattus norvegicus similar to g... 44 0.013
gi|34877741|ref|XM_341422.1| Rattus norvegicus similar to g... 44 0.013
gi|34875552|ref|XM_224472.2| Rattus norvegicus similar to g... 44 0.013
gi|34875520|ref|XM_224464.2| Rattus norvegicus similar to g... 44 0.013
gi|34852784|ref|XM_342166.1| Rattus norvegicus similar to g... 44 0.013
gi|34879625|ref|XM_223717.2| Rattus norvegicus similar to g... 44 0.013
gi|34861964|ref|XM_219750.2| Rattus norvegicus similar to g... 44 0.013
gi|34877345|ref|XM_214046.2| Rattus norvegicus similar to g... 44 0.013
gi|34875902|ref|XM_213991.2| Rattus norvegicus similar to g... 44 0.013
gi|34879481|ref|XM_213867.2| Rattus norvegicus similar to g... 44 0.013
gi|34879275|ref|XM_232707.2| Rattus norvegicus similar to g... 44 0.013
gi|37590766|gb|BC059110.1| Rattus norvegicus glyceraldehyde... 44 0.013
gi|33942196|gb|AC102494.6| Mus musculus chromosome 7, clone... 44 0.013
gi|34368616|gb|AC125565.5| Rattus norvegicus 2 BAC CH230-9H... 44 0.013
gi|21218401|gb|AF512009.1| Sigmodon hispidus glyceraldehyde... 44 0.013
gi|27356781|gb|AY066007.1| Meriones unguiculatus glyceralde... 44 0.013
gi|56187|emb|X02231.1|RNGADPHR Rattus norvegicus mRNA for g... 44 0.013
gi|32452393|dbj|AB040445.1| Meriones unguiculatus GAPDH mRN... 44 0.013
gi|28189708|dbj|AB098979.1| Bos taurus mRNA for similar to ... 44 0.013
gi|28189618|dbj|AB098934.1| Bos taurus mRNA for similar to ... 44 0.013
gi|28189564|dbj|AB098907.1| Bos taurus mRNA for similar to ... 44 0.013
gi|2285902|emb|AJ000039.1|BTGAPDH Bos taurus mRNA for glyce... 44 0.013
gi|1841757|gb|U85042.1|BTU85042 Bos taurus glyceraldehyde-p... 44 0.013
gi|10190788|gb|AF106860.2|AF106860 Rattus norvegicus glycer... 44 0.013
gi|9798637|dbj|AB017801.1| Rattus norvegicus mRNA for glyce... 44 0.013
gi|1675348|gb|U75401.1|RNU75401 Rattus norvegicus glycerald... 44 0.013
gi|31377487|ref|NM_017008.2| Rattus norvegicus glyceraldehy... 44 0.013
gi|22137354|gb|BC024703.1| Mus musculus RIKEN cDNA 3000002C... 42 0.051
gi|34870654|ref|XM_340786.1| Rattus norvegicus similar to g... 42 0.051
gi|34865813|ref|XM_343455.1| Rattus norvegicus similar to g... 42 0.051
gi|34867135|ref|XM_218090.2| Rattus norvegicus similar to g... 42 0.051
gi|34879837|ref|XM_217067.2| Rattus norvegicus similar to g... 42 0.051
gi|34933053|ref|XM_233295.2| Rattus norvegicus similar to g... 42 0.051
gi|34852539|ref|XM_215391.2| Rattus norvegicus similar to g... 42 0.051
gi|34852531|ref|XM_342144.1| Rattus norvegicus similar to g... 42 0.051
gi|34857537|ref|XM_215798.2| Rattus norvegicus similar to g... 42 0.051
gi|34879542|ref|XM_341616.1| Rattus norvegicus similar to G... 42 0.051
gi|34858143|ref|XM_227423.2| Rattus norvegicus similar to g... 42 0.051
gi|34855494|ref|XM_215526.2| Rattus norvegicus similar to G... 42 0.051
gi|34853006|ref|XM_215441.2| Rattus norvegicus similar to g... 42 0.051
gi|34879714|ref|XM_223741.2| Rattus norvegicus similar to g... 42 0.051
gi|34880932|ref|XM_213945.2| Rattus norvegicus similar to g... 42 0.051
gi|45120379|gb|AC147251.3| Mus musculus BAC clone RP24-114L... 42 0.051
gi|45237291|gb|AC140286.3| Mus musculus BAC clone RP24-573E... 42 0.051
gi|28189720|dbj|AB098985.1| Bos taurus mRNA for similar to ... 42 0.051
gi|39752877|gb|AC123950.3| Mus musculus BAC clone RP23-162I... 42 0.051
gi|204248|gb|M17701.1|RATGAPDHA Rat glyceraldehyde-3-phosph... 42 0.051
gi|21531039|emb|AL645640.14| Mouse DNA sequence from clone ... 42 0.051
gi|38327333|gb|AY363102.1|AY363102S1 Mus musculus diadenosi... 40 0.20
gi|37574271|gb|AC146815.1| Mus musculus BAC clone RP23-239A... 40 0.20
gi|38371912|gb|AC101886.16| Mus musculus chromosome 15, clo... 40 0.20
gi|27356748|gb|AC121959.3| Mus musculus BAC clone RP24-247N... 40 0.20
gi|29373194|gb|AC125177.4| Mus musculus BAC clone RP23-315H... 40 0.20
gi|22138613|gb|AC121838.2| Mus musculus BAC clone RP24-74O1... 40 0.20
gi|38173991|gb|BC061210.1| Mus musculus cDNA clone IMAGE:30... 40 0.20
gi|38092293|ref|XM_354654.1| Mus musculus similar to glycer... 40 0.20
gi|33569140|emb|AL845336.9| Mouse DNA sequence from clone R... 40 0.20
gi|25815334|gb|AC091683.52| Mus musculus chromosome 13 clon... 40 0.20
gi|46359985|gb|AC131323.9| Mus musculus chromosome 17, clon... 40 0.20
gi|46359983|gb|AC124113.9| Mus musculus chromosome 5, clone... 40 0.20
gi|33146035|emb|AL954379.17| Mouse DNA sequence from clone ... 40 0.20
gi|45356249|gb|AC121279.7| Mus musculus chromosome 3, clone... 40 0.20
gi|31043743|emb|AL831714.14| Mouse DNA sequence from clone ... 40 0.20
gi|30140547|emb|AL935328.14| Mouse DNA sequence from clone ... 40 0.20
gi|28625316|emb|AL929179.6| Mouse DNA sequence from clone R... 40 0.20
gi|25013342|gb|AC087416.18| Mus musculus chromosome 2 clone... 40 0.20
gi|41470583|gb|AC115705.13| Mus musculus chromosome 3, clon... 40 0.20
gi|41393315|gb|AC105297.35| Mus musculus clone rp23-14k19 m... 40 0.20
gi|40352726|gb|BC064681.1| Mus musculus similar to glyceral... 40 0.20
gi|41054967|ref|NM_199472.1| Mus musculus similar to glycer... 40 0.20
gi|24474460|emb|AL672180.11| Mouse DNA sequence from clone ... 40 0.20
